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IP05 Protein G Plus/Protein A Agarose Suspension

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IP05
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Overview

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IP05-1.5ML
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      Description
      OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
      Catalogue NumberIP05
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid slurry
      Formulation33% slurry in PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (see comments)
      Application CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein G Plus/Protein A Agarose Suspension SDS

      Title

      Safety Data Sheet (SDS) 

      Protein G Plus/Protein A Agarose Suspension Certificates of Analysis

      TitleLot Number
      IP05

      Citations

      Title
    • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
    • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
    • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2008 JSW
      ApplicationImmunoprecipitation (see comments)
      DescriptionProtein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
      BackgroundProtein G PLUS/Protein A-Agarose mixture provides superior performance for binding of most species and subclasses of immunoglobulins. For use with rat IgM antibodies, use with goat second-step antibodies.
      FormLiquid slurry
      Formulation33% slurry in PBS.
      Preservative≤0.1% sodium azide
      CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      Citation
    • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
    • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
    • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
    • Related Products & Applications

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
      Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification