539305 Protein G, Alkaline Phosphatase Conjugate

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539305
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539305-500UG
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      Plastic ampoule 500 μg
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      Description
      OverviewProtein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
      Catalogue Number539305
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application NotesDirect Immunoassay (1:2500)
      Application Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PuritySingle band at 33.8 kDa by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein G, Alkaline Phosphatase Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Protein G, Alkaline Phosphatase Conjugate Certificates of Analysis

      TitleLot Number
      539305
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-April-2009 RFH
      ApplicationDirect Immunoassay (1:2500)
      DescriptionRecombinant protein G, a cell-surface protein of Streptococcus species, binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economical means to isolate, detect and purify antibodies produced by many animals. It is especially valued because of its ability to react with human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e., bovine goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE, and IgM. Useful for detection of primary antibodies by various immunoassay methods at an average dilution of 1:2500.
      FormLiquid
      FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      PuritySingle band at 33.8 kDa by SDS-PAGE
      Preservative≤0.1% sodium azide
      Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling