16-157 Protein A Agarose/Salmon Sperm DNA, 2.5 mL

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16-157
2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
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      Overview

      Replacement Information

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      Achieve first time ChIP success with EZ-ChIP Assays
      Description
      Catalogue Number16-157
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionProtein A Agarose/Salmon Sperm DNA, 2.5 mL
      OverviewProtein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
      Alternate Names
      • ChIP agarose beads
      • ChIP agaraose A beads
      Background InformationProtein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
      References
      Product Information
      Presentation10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationRecombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.
      Application NotesSuitable for use in chromatin immunoprecipitation (ChIPs) assays
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C
      Packaging Information
      Material Size2.5 mL
      Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein A Agarose/Salmon Sperm DNA, 2.5 mL SDS

      Title

      Safety Data Sheet (SDS) 

      Protein A Agarose/Salmon Sperm DNA, 2.5 mL Certificates of Analysis

      TitleLot Number
      Protein A agarose/Salmon Sperm DNA - 06070344700607034470
      Protein A agarose/Salmon Sperm DNA - 06080381930608038193
      Protein A agarose/Salmon Sperm DNA - 07010493850701049385
      Protein A agarose/Salmon Sperm DNA - 1712417124
      Protein A agarose/Salmon Sperm DNA - 1811018110
      Protein A agarose/Salmon Sperm DNA - 1916019160
      Protein A agarose/Salmon Sperm DNA - 1962019620
      Protein A agarose/Salmon Sperm DNA - 2077120771
      Protein A agarose/Salmon Sperm DNA - 2115121151
      Protein A agarose/Salmon Sperm DNA - 2189821898

      References

      Reference overviewApplicationPub Med ID
      A novel HSI2 mutation in Arabidopsis affects the PHD-like domain and leads to derepression of seed-specific gene expression.
      Vijaykumar Veerappan,Jing Wang,Miyoung Kang,Joohyun Lee,Yuhong Tang,Ajay K Jha,Huazhong Shi,Ravishankar Palanivelu,Randy D Allen
      Planta  236  2012

      Show Abstract
      22476218 22476218
      The adenovirus L4-22K protein is multifunctional and is an integral component of crucial aspects of infection.
      Wu, Kai, et al.
      J. Virol., 86: 10474-83 (2012)  2012

      Show Abstract
      22811519 22811519
      Methods for analyzing histone citrullination in chromatin structure and gene regulation.
      Pingxin Li,Jing Hu,Yanming Wang
      Methods in molecular biology (Clifton, N.J.)  809  2012

      Show Abstract
      22113295 22113295
      In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.
      Foster JG, Cassida KA, Turner KE
      Vet Parasitol  2011

      Show Abstract
      21477927 21477927
      The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma.
      Matthew Bott,Marie Brevet,Barry S Taylor,Shigeki Shimizu,Tatsuo Ito,Lu Wang,Jenette Creaney,Richard A Lake,Maureen F Zakowski,Boris Reva,Chris Sander,Robert Delsite,Simon Powell,Qin Zhou,Ronglai Shen,Adam Olshen,Valerie Rusch,Marc Ladanyi
      Nature genetics  43  2011

      Show Abstract
      21642991 21642991
      A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
      Du, JX; McConnell, BB; Yang, VW
      The Journal of biological chemistry  285  28298-308  2010

      Show Abstract
      20584900 20584900
      Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
      Lauren J Buro,Shaili Shah,Melissa A Henriksen
      Journal of visualized experiments : JoVE  2010

      Show Abstract
      20729799 20729799
      Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
      Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
      The Journal of biological chemistry  285  5405-16  2010

      Show Abstract Full Text Article
      20007975 20007975
      The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation.
      Ralf Stracke,Jean-Jacques Favory,Henriette Gruber,Lutz Bartelniewoehner,Sebastian Bartels,Melanie Binkert,Markus Funk,Bernd Weisshaar,Roman Ulm
      Plant, cell & environment  33  2010

      Show Abstract
      19895401 19895401
      Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo.
      Monica E Ferreira, Philippe Prochasson, Kurt D Berndt, Jerry L Workman, Anthony P H Wright
      The FEBS journal  276  2557-65  2009

      Show Abstract
      19476494 19476494

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)