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539134 Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem

Overview

Replacement Information

Key Specifications Table

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539134-10ML
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      539134-1EA
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          539134-1ML
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              539134-1SET
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                  1 set
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                  Description
                  OverviewA cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cell and tissue extracts, but is also suitable for bacterial extracts. Supplied with a data sheet.
                  Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
                  Catalogue Number539134
                  Brand Family Calbiochem®
                  Application Data
                  References
                  Product Information
                  FormLiquid
                  ReversibleN
                  Applications
                  Biological Information
                  Primary TargetAspartic, cysteine, and serine proteases as well as aminopeptidases
                  Physicochemical Information
                  Cell permeableN
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  R PhraseR: 36/37/38

                  Irritating to eyes, respiratory system and skin.
                  S PhraseS: 26-36

                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Dry Ice Only
                  Toxicity Irritant
                  Storage -20°C
                  Do not freeze Ok to freeze
                  Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem Certificates of Analysis

                  TitleLot Number
                  539134

                  Brochure

                  Title
                  Bulk packaging
                  Protein Purification and Detection Tools

                  Citations

                  Title
                • Hofer, M.J., et al. 2010. J. Neurosci. 30, 1149.
                • Diao, A., et al. 2008. Journal of Biological Chemistry 283, 6957.
                • Lloyd-Burton, S.M., et al. 2007. Journal of Biological Chemistry 282, 9526.
                • Wittmann, B.M., et al. 2007. Cancer Research 67, 9549.
                • Horowitz, J.C., et al. 2006. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L415.
                • Mattos-Graner, R.O., et al. 2006. Journal of Bacteriology 188, 3813.
                • Unoki, M., et al. 2006. Journal of Biological Chemistry 281, 34677.
                • White, A.R., et al. 2006. journal of Biological Chemistry 281, 17670.
                • Choudhury, R., et al. 2005. Molecular Biology of the Cell 16, 3467.
                • Georgescu, S.P. et al. 2005. Molecular Endocrinology 19, 2491.
                • Han, J., et al. 2005. Journal of Biological Chemistry 280, 16383.
                • Liedtke, C.M., et al. 2005. Journal of Biological Chemistry 280, 25491.
                • Taniuchi, K., et al. 2005. Cancer Research 65, 105.
                • Abdolzade-Bavil, A., et al. 2004. Proteomics 4, 1397.
                • Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision27-February-2012 RFH
                  Application Data
                  DescriptionThis protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
                  FormLiquid
                  Recommended reaction conditionsOne ml is sufficient for approximately 20 g tissue. Alternatively, depending on the application, it can be used effectively with a dilution factor in the range of 1:100-1:2000. The optimal working concentration should be determined for each individual application. A dilution of 1:200 typically works well as a starting concentration.
                  Storage -20°C
                  Do Not Freeze Ok to freeze
                  Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
                  Toxicity Irritant
                  Citation
                • Hofer, M.J., et al. 2010. J. Neurosci. 30, 1149.
                • Diao, A., et al. 2008. Journal of Biological Chemistry 283, 6957.
                • Lloyd-Burton, S.M., et al. 2007. Journal of Biological Chemistry 282, 9526.
                • Wittmann, B.M., et al. 2007. Cancer Research 67, 9549.
                • Horowitz, J.C., et al. 2006. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L415.
                • Mattos-Graner, R.O., et al. 2006. Journal of Bacteriology 188, 3813.
                • Unoki, M., et al. 2006. Journal of Biological Chemistry 281, 34677.
                • White, A.R., et al. 2006. journal of Biological Chemistry 281, 17670.
                • Choudhury, R., et al. 2005. Molecular Biology of the Cell 16, 3467.
                • Georgescu, S.P. et al. 2005. Molecular Endocrinology 19, 2491.
                • Han, J., et al. 2005. Journal of Biological Chemistry 280, 16383.
                • Liedtke, C.M., et al. 2005. Journal of Biological Chemistry 280, 25491.
                • Taniuchi, K., et al. 2005. Cancer Research 65, 105.
                • Abdolzade-Bavil, A., et al. 2004. Proteomics 4, 1397.
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                  Categories

                  Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Proteases Inhibitors > Protease Inhibitor Cocktails