Millipore Sigma Vibrant Logo

ProSep® Ultra Plus Chromatography Media

Designed for high throughput affinity purification of monoclonal antibodies and other proteins.



Ordering Information

ProSep® Ultra Plus Chromatography Media Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Uniticon Pack Sizeicon
175118822Prosep® Ultra Plus 2 mL Bottle 1 x 2 mL Show Pricing & Availability
175118824Prosep® Ultra Plus 10 mL Bottle 1 x 10 mL Show Pricing & Availability
175118827Prosep® Ultra Plus 100 mL Bottle 1 x 100 mL Show Pricing & Availability
175118830Prosep® Ultra Plus 1 L Bottle 1 x 1 L Show Pricing & Availability
175118833Prosep® Ultra Plus 5 L Bottle 1 x 5 L Show Pricing & Availability
175118834Prosep® Ultra Plus 25 L Bottle 1 x 25 L Show Pricing & Availability
175118835Prosep® Ultra Plus 10 L Bottle 1 x 10 L Show Pricing & Availability

Supplied as 50% slurry in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol.

Back To Top

Columns Clear Sorting & Filtering
Catalog Numbericon Descriptionicon
125067MiniChrom ProSep® Ultra Plus, 1 ml Show Pricing & Availability
125076MiniChrom Column ProSep® Ultra Plus, 5 ml Show Pricing & Availability
125135RoboColumn ProSep® Ultra Plus, 0.2 ml Show Pricing & Availability
125143RoboColumn ProSep® Ultra Plus, 0.6 ml Show Pricing & Availability

Supplied in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol.

Back To Top



    Biochromatography Portfolio Guide
    Chromatography Purification Guide (EMD)
    ProSep® Ultra Plus Chromatography Media (EMD)
    Provantage® Services - Chromatography Column Packing Services

    Technical Info

    Benzyl alcohol clearance from Protein A resins - EMD version
    Consistent Packing Performance for Diverse Media Types with QuikScale® Biochromatography Columns, TB35290000
    ProSep® A Chromatography Resins: Virus Clearance and Sanitization
    Technical Brief - Citric acid or acetic acid? (EMD)

    Data Sheet

    Mobius® FlexReady Solution with Smart Flexware™ Assemblies for Chromatography and TFF
    ProSep® Ultra Plus Chromatography Media (MilliporeSigma)


    HCP clearance strategies for Protein A chromatography
    Simple process strategies to increase the utilization of Protein A media in clinical manufacturing


    Reference overviewApplication
    Determining the Useful Lifetime of Chromatography Resins: Prospective Small-Scale Studies
    O’Leary et al.,Biopharm, September 2001, pp 10 -18
    Biopharm, September 2001, pp 10 -18  2001

    Pressure-flow relationships for packed beds of compressible chromatography media at laboratory and production scale
    Stickel J.J.; Fotopoulos A.
    Biotechnology Progress, 17/4 (744-751), 2001  2001

    Process development studies
    Determining the useful lifetime chromatography resins: Prospective small-scale studies
    O'Leary R.M.; Feuerhelm D.; Peers D.; Xu Y.; Blank G.S.
    BioPharm ( BIOPHARM ) (United States) 2001, 14/9 (10-18)  2001

    Process development studies
    Affinity chromatography is vital to protein research and development

    Process development studies
    Industrial purification of pharmaceutical antibodies: development, operation, and validation of chromatography processes.
    Fahrner RL, Knudsen HL, Basey CD, Galan W, Feuerhelm D, Vanderlaan M, Blank GS.
    Biotechnol Genet Eng Rev 2001;18:301-27  2001

    Process development studies
    Preparative Immunoaffinity Techniques
    Michael Price and Kami Beyzavi; Peptide Antigens, A Practical Approach, Chapter 5
    Peptide Antigens, A Practical Approach, Chapter 5  1999

    Performance comparison of Protein A affinity chromatography adsorbents for purifying recombinant monoclonal antibodies
    Robert L Fahrner, Duncan H Whitney, Martin Vanderlaan, Gregory S Blank.
    Biotechnol. Appl. Biochem, Vol 30 121-128, 1999  1999

    Performance comparison of Protein A affinity-chromatography sorbents for purifying recombinant monoclonal antibodies
    Fahrner, et al.,Biotechnol. Appl. Biochem. (1999) 30, 121-128
    Biotechnol. Appl. Biochem. (1999) 30, 121-128  1999

    The optimal flow rate and column length for maximum production rate of Protein A affinity chromatography
    R.L Fahrner, H.V. Iyer, G.S. Blank
    Bioprocess Engineering 21 (1999) 287-292 - Springer-Verlag.  1999

    Process development studies
    Development, validation and application of an ultra-sensitive two-site enzyme immunoassay for human follistatin
    LW Evans, S Muttukrishna and NP Groome
    Journal of Endocrinology, 156, 275-282, 1998  1998


    What is the difference between ProSep-vA Ultra and ProSep-vA High Capacity chromatography media?Prosep-vA Ultra uses a 700 angstrom pore size porous glass and ProSep vA High Capacity uses a 1000 Angstrom porous glass. The Prosep-vA Ultra has a greater surface area due to the smaller pore size, which allows the immobilization of a larger amount of protein A. The increased amount of protein A results in a higher capacity resin.
    How do I choose between ProSep-vA Ultra versus ProSep-vA High Capacity?Prosep-vA Ultra has a higher binding capacity than ProSep-vA High Capacity - ≥ 56mg/ml vs ≥ 40 mg/ml.

    ProSep-vA High Capacity has a larger pore size and may perform better when purifying fusion proteins or antibody conjugates with molecular weights above 150 kD.
    What is the shelf life of the Prosep-A family of protein A affinity media?One Year. Shelf life studies are ongoing and will be increased as data becomes available. Please contact Technical Service for further shelf life information.
    What is Prosep-vA ?ProSep-vA is native protein A derived from Staphylococcus aureus which has been immobilized onto porous glass beads. ProSep-vA is available on glass of two diffferent porosities - ProSep-vA ultra (700 angstroms) and ProSep-vA High Capacity (1000 angstroms)
    Do you provide reguatory support?A regulatory reference document is available for ProSep-vA High Capacity and will soon be available for Prosep-vA Ultra.

    As the raw materials and the chemistry used in the manufacture of ProSep-vA Ultra are the same as for ProSep-vA High Capacity , the regulators will be familiar with the product. ProSep-vA High Capacity has been and is widely used in various licensed MAb products and products in clinical trails.

    User Guides

    Affinity Chromatography Media

    Related Products & Applications

    Product Families

    Related Products By: Application Facete

    Related Products By: Brand Facete


    Biopharmaceutical Manufacturing > Downstream Processing > Chromatography > Affinity Chromatography