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PI-12K Porcine Insulin RIA

PI-12K
250 tubes  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Insulin PoRIARadioactive 125I
      Description
      Catalogue NumberPI-12K
      DescriptionPorcine Insulin RIA
      Background InformationInsulin is a 5.8 kD hormone is secreted by the islet cells in the pancreas.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 7 ± 0.6 µU/ml
      • ED50 = 30 ± 1.2 µU/ml
      • ED20 = 126 ± 3.0 µU/ml
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
      Key Applications
      • Radioimmunoassay
      Application NotesOvernight, equilibrium incubation at 4°C. Sample volume < 100 μl serum, plasma, or tissue culture media.
      Biological Information
      SpecificityPorcine Insulin 100% Procine Proinsulin 53% Human Insulin 100% Bovine Insulin 90% Bovine Proinsulin 50% Intact Human Proinsulin 38% Rat Insulin 19% Des 31, 32 HPI 47% Des 64, 65 HPI 72% Glucagon Not Detectable Pancreatic Polypeptide Not Detectable Somatos
      Species Reactivity
      • Pig
      Analytes Available
      • Insulin
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Radioactive Isotype125I-Insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Physicochemical Information
      Standard Curve Range
      • 2–200 µU/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Porcine Insulin RIA Kit

      Porcine Insulin RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Cereal type and heat processing of the cereal affect nutrient digestibility and dynamics of serum insulin and ghrelin in weanling pigs.
      Menoyo D, Serrano MP, Barrios V, Valencia DG, Lázaro R, Argente J, Mateos GG
      Journal of animal science  2011

      Show Abstract
      21478449 21478449
      Gene expression profiling of the short-term adaptive response to acute caloric restriction in liver and adipose tissues of pigs differing in feed efficiency.
      Lkhagvadorj S, Qu L, Cai W, Couture OP, Barb CR, Hausman GJ, Nettleton D, Anderson LL, Dekkers JC, Tuggle CK
      American journal of physiology. Regulatory, integrative and  298  R494-R507  2010

      Show Abstract
      19939971 19939971
      Postprandial response of plasma insulin, amylin and acylated ghrelin to various test meals in lean and obese cats.
      Martin LJ, Siliart B, Lutz TA, Biourge V, Nguyen P, Dumon HJ
      The British journal of nutrition  2010

      Show Abstract
      20100379 20100379
      Performance and metabolic and endocrine changes with emphasis on glucose metabolism in high-yielding dairy cows with high and low fat content in liver after calving.
      H M Hammon, G Stürmer, F Schneider, A Tuchscherer, H Blum, T Engelhard, A Genzel, R Staufenbiel, W Kanitz
      Journal of dairy science  92  1554-66  2009

      Show Abstract
      19307636 19307636
      Metabolic changes and net portal flux in dairy cows fed a ration containing rumen-protected fat as compared to a control diet.
      H M Hammon,C C Metges,P Junghans,F Becker,O Bellmann,F Schneider,G Nürnberg,P Dubreuil,H Lapierre
      Journal of dairy science  91  2008

      Show Abstract
      18096942 18096942
      Acute metabolic responses of postpartal dairy cows to subcutaneous glucagon injections, oral glycerol, or both.
      M A Osman, P S Allen, N A Mehyar, G Bobe, J F Coetzee, K J Koehler, D C Beitz
      Journal of dairy science  91  3311-22  2008

      Show Abstract
      18765590 18765590
      Induction of hyperlipidemia by intravenous infusion of tallow emulsion causes insulin resistance in Holstein cows.
      J A A Pires, A H Souza, R R Grummer
      Journal of dairy science  90  2735-44  2007

      Show Abstract
      17517713 17517713
      Gonadectomy and high dietary fat but not high dietary carbohydrate induce gains in body weight and fat of domestic cats.
      Robert C Backus, Nick J Cave, Duane H Keisler
      The British journal of nutrition  98  641-50  2007

      Show Abstract
      17524182 17524182
      Glucose-dependent insulin response and milk production in heifers within a segregating resource family population.
      H M Hammon, O Bellmann, J Voigt, F Schneider, C Kühn
      Journal of dairy science  90  3247-54  2007

      Show Abstract
      17582108 17582108
      Comparing mRNA levels of genes encoding leptin, leptin receptor, and lipoprotein lipase between dairy and beef cattle.
      M Q Ren, J Wegner, O Bellmann, G A Brockmann, F Schneider, F Teuscher, K Ender
      Domestic animal endocrinology  23  371-81  2002

      Show Abstract
      12206871 12206871

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Analyte Quarterly Vol. 2 2020
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges for Catalog # 6000-K Expected Ranges of Each Analyte Using EMD MILLIPORE RIA Kits as Listed Below

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits