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71296 PhosphoSafe™ Extraction Reagent

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71296
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Overview

Replacement Information

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Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
71296-3
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      Plastic ampoule 25 ml
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      71296-4
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          Glass bottle 125 ml
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          Description
          OverviewPhosphoSafe™ Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster™ Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).


          Catalogue Number71296
          Brand Family Novagen®
          Features and benefits
          • Phosphorylation state preserved during extraction
          • Compatible with kinase assays
          References
          ReferencesTrim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.
          Product Information
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Toxic
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoSafe™ Extraction Reagent SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoSafe™ Extraction Reagent Certificates of Analysis

          TitleLot Number
          71296

          References

          Reference overview
          Trim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.

          Technical Info

          Title
          Western Blotting Quick Reference Product Guide

          Citations

          Title
        • Yanxia Liu, et al. (2007) A genomic screen for activators of the antioxidant response element. Procedings of the National Academy of Science 104, 5205-5210.
        • Elena Martinelli, et al. (2007) HIV-1 gp120 inhibits TLR9-mediated activation and IFN-α secretion in plasmacytoid dendritic cells. Procedings of the National Academy of Science 104, 3396-3401.
        • Dagmar Pommereit and Fred S. Wouters. (2007) An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth. Journal of Cell Science 120, 2694-2705.
        • Aicha Saadane, et al. (2007) Parthenolide inhibits IκB kinase, NF-κB activation, and inflammatory response in cystic fibrosis cells and mice. American Journal of Respiratory Cell and Molecular Biology 36, 728-736.
        • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by Varicella-Zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
        • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by varicella-zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
        • Marisol Quintero, et al. (2006) Mitochondria as signaling organelles in the vascular endothelium. Proceedings of the National Academy of Sciences (USA) 103, 5379-5384.
        • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
        • Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
        • Ralf Gutzmer, et al. (2005) Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells. Journal of Immunology 5224-5232.
        • Mauro Cesar Isoldi, et al. (2005) Rhabdomeric phototransduction initiated by the vertebrate photopigment melanopsin. Procedings of the National Academy of Science 102, 1217-1221.
        • Elad Katz, et al. (2005) MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three-dimensional culture. Journal of Experimental Medicine 201, 431-439.
        • Hiroki Kuroda, et al. (2005) Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation. Genes and Development 19, 1022-1027.
        • Markus Utech, et al. (2005) Mechanism of IFN-γ-Induced Endocytosis of Tight Junction Proteins: Mysoin II-Dependent Vacuolarization of the Apical Plasma Membrane. Molecular Biology of the Cell 16, 5040-5052.
        • User Protocols

          Title
          TB402 PhosphoSafe™ Extraction Reagent