506119 PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ca, H, M, R Rb Polyclonal Antibody

Pricing & Availability

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506119-10T
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      Description
      OverviewRecognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
      Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number506119
      Brand Family Calbiochem®
      Application Data
      Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
      References
      ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
      Nakagami, H., et al. 2001. Diabetes 50, 1472.
      Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
      Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
      Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
      Han, J., et al. 1994. Science 265, 808.
      Lee, J.C., et al. 1994. Nature 372, 739.
      Rouse, J., et al. 1994. Cell 78, 1027.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb Certificates of Analysis

      TitleLot Number
      506119

      References

      Reference overview
      Marinissen, M.J., et al. 2001. Genes Dev. 15, 535.
      Nakagami, H., et al. 2001. Diabetes 50, 1472.
      Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
      Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
      Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
      Han, J., et al. 1994. Science 265, 808.
      Lee, J.C., et al. 1994. Nature 372, 739.
      Rouse, J., et al. 1994. Cell 78, 1027.

      Brochure

      Title
      MAPK Pathway Poster ( 750 KB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application Data
      Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
      Backgroundp38 MAP kinase (MAPK), also called RK and CSBP, is the mammalian homologue of yeast HOG kinase and participates in a cascade that controls cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light, and growth factors. Both MKK3 and SEK phosphorylate p38 on tyrosine and threonine resulting in p38 activation. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
      IsotypeIgG
      Speciescanine, human, mouse, rat
      Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
      Nakagami, H., et al. 2001. Diabetes 50, 1472.
      Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
      Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
      Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
      Han, J., et al. 1994. Science 265, 808.
      Lee, J.C., et al. 1994. Nature 372, 739.
      Rouse, J., et al. 1994. Cell 78, 1027.

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