PS1013 PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

PS1013
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M Rb Polyclonal Antibody

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PS1013-10T
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      Description
      OverviewRecognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
      Catalogue NumberPS1013
      Brand Family Calbiochem®
      SynonymsAnti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
      Application Data
      Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
      References
      ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
      Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
      Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
      Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
      Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PS1013

      References

      Reference overview
      Meyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
      Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
      Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
      Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      SynonymsAnti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~200 kDa VEGFR2 protein phosphorylated at Tyr1054/1059.
      BackgroundVascular Endothelial Growth Factor Receptor 2 (VEGFR2) is receptor tyrosine kinase that serves as a key mediator in angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 (FMS-like tyrosine kinase 1) and Flt-4 (FMS-like tyrosine kinase 4). Upon binding to its ligand, VEGF, VEGFR2 undergoes ligand-induced dimerization, which activates its intrinsic tyrosine kinase activity resulting in auto- and trans-phosphorylation on specific tyrosine residues in the cytoplasmic domain. These tyrosine residues, when phosphorylated, are involved as docking sites to recruit molecules containing Src homology 2 (SH2) or phosphotyrosine-binding domains and to convey signals to downstream effectors. Phosphorylation of VEGFR2 at Tyr1054 and Tyr1059 within the activation loop is required for required for activation of VEGFR2 and enhances its intrinsic tyrosine kinase activity. Phosphorylation at Tyr1059 is also responsible for VEGF-induced intracellular Ca2+ mobilization.
      HostRabbit
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
      IsotypeIgG
      Speciesmouse
      Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
      Preservative≤0.1% sodium azide
      CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
      Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
      Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
      Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.