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525290 PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb

This PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Phosphotyrosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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525290
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesRbPolyclonal Antibody

Pricing & Availability

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525290-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts.
      Catalogue Number525290
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (1:5000-1:10,000)
      Immunoblotting (1:1000-1:2000)
      Immunoprecipitation (2-5 µg)
      Application CommentsDo not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified phosphotyrosine conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Identification Number
      Catalog Number GTIN
      525290-100UG4055977270679

      Documentation

      PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb Certificates of Analysis

      TitleLot Number
      525290
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-December-2007 JSW
      ApplicationELISA (1:5000-1:10,000)
      Immunoblotting (1:1000-1:2000)
      Immunoprecipitation (2-5 µg)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes phosphotyrosine-containing proteins.
      BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
      HostRabbit
      Immunogenpurified phosphotyrosine conjugated to KLH
      IsotypeIgG
      Speciesa broad range of species
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDo not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling