525295 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of Species M Monoclonal Antibody

Pricing & Availability

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525295-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
      Catalogue Number525295
      Brand Family Calbiochem®
      References
      ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Product Information
      FormLiquid
      FormulationIn 10 mM sodium phosphate, pH 7.4.
      Positive controlAny cell lysate or tissue extract
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.
      Key Applications Immunocytochemistry
      Immunoprecipitation
      Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1:500-1:1000, see application references)
      Frozen Sections (see application references)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see comments)
      Application CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
      ClonePY20
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Certificates of Analysis

      TitleLot Number
      525295

      References

      Reference overview
      Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-December-2007 JSW
      ApplicationELISA (see comments)
      Immunoblotting (1:500-1:1000, see application references)
      Frozen Sections (see application references)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
      BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
      HostMouse
      ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
      ClonePY20
      IsotypeIgG2b
      Speciesa broad range of species
      Positive controlAny cell lysate or tissue extract
      FormLiquid
      FormulationIn 10 mM sodium phosphate, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Application referencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.