PK1008 Sigma-AldrichPhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

Safety Data Sheet (SDS) 

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	16-October-2007 RFH
Synonyms	Anti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
Application	Flow Cytometry (1:200) Immunoblotting (1:1000) Immunoprecipitation (1:50) Paraffin Sections (1:50, heat pre-treatment required; see comments)
Application Data	Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence. Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain. Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.
Description	Rabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751.
Background	The PDGF family of growth factors consists of several disulfide-linked dimeric isoforms-PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD-that bind in distinct patterns to the specific receptor tyrosine kinases, PDGFR-α and PDGFR-β. PDGFR-α can bind to all isoforms of PDGF except PDGF-DD and PDGFR-β only binds to PDGF-DD. Both receptors can form homo- and heterodimers, including heterodimer formation with EGFR, which can also be stimulated by PDGF. Similar to other receptor tyrosine kinases, ligand binding leads to receptor dimerization and autophosphorylation, which permits binding and activatino of cytoplasmic SH2 domain-containing signal transduction molecules, including Grb2, Src, GAP, PI-3K, PLCγ, and Nck. As a result, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, cell migration, and cell differentiation. Tyr751 of PDGFR-β is the docking site for PI-3K. Phosphorylated pentapeptides derived from Tyr751 are reported to inhibit the association of PI-3K with PDGFR-β.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
Isotype	IgG1
Species	human, mouse, rat
Positive control	Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
Form	Liquid
Formulation	In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preservative	None
Comments	For staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Do not aliquot.
Toxicity	Standard Handling
References	Betsholtz, C., et al. 2001. Bioessays 23, 494. Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1. Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263. Panyotou, G. et al. 1992. EMBO J. 11, 4261. Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39. Deuel, T.F., et al. 1988. Biofactors 1, 213.