PC635 PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

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PC635-50UL
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      Plastic ampoule 50 ul
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      Description
      OverviewRecognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
      Catalogue NumberPC635
      Brand Family Calbiochem®
      SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
      References
      ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
      Lim, D.S., et al. 2000. Nature 404, 613.
      Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
      Zhong, Q., et al. 1999. Science 285, 747.
      Carney, J.P., et al. 1998. Cell 93, 477.
      Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
      Varon, R., et al. 1998. Cell 93, 467.
      Product Information
      FormLiquid
      FormulationSerum diluted in PBS.
      Positive controlγ-irradiated lymphoblastoid cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (1:16,000)
      Immunoblotting (1:1000)
      Immunoprecipitation (1 µl/500 µg cell extract)
      Application CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC635

      References

      Reference overview
      Gatei, M., et al. 2000. Nat. Genet. 25, 115.
      Lim, D.S., et al. 2000. Nature 404, 613.
      Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
      Zhong, Q., et al. 1999. Science 285, 747.
      Carney, J.P., et al. 1998. Cell 93, 477.
      Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
      Varon, R., et al. 1998. Cell 93, 467.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-October-2007 RFH
      SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
      ApplicationELISA (1:16,000)
      Immunoblotting (1:1000)
      Immunoprecipitation (1 µl/500 µg cell extract)
      DescriptionRabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
      BackgroundNibrin, a ~95 kDa double stranded DNA repair protein, is mutated in Nijmegen breakage syndrome (NBS). Patients with NBS exhibit chromosomal instability, resulting in hypersensitivity to irradiation and cancer predisposition. Nibrin forms a manganese-sensitive complex with hMre11 and hRAD50 that has single strand DNA endonuclease and exonuclease activities and is likely to be important for DNA excision, repair, and stability. This nibrin/Mre11/RAD50 complex is inhibited by BRCA1. The ATM (ataxia telangiectasia mutated) kinase has been shown to phosphorylate nibrin in an S-phase checkpoint pathway. Nibrin levels are elevated in testis tissue, suggesting a possible role in meiotic recombination. Nibrin may also be involved in telomere length maintenance.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
      IsotypeIgG
      Specieshuman
      Positive controlγ-irradiated lymphoblastoid cells
      FormLiquid
      FormulationSerum diluted in PBS.
      PreservativeNone
      CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
      Lim, D.S., et al. 2000. Nature 404, 613.
      Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
      Zhong, Q., et al. 1999. Science 285, 747.
      Carney, J.P., et al. 1998. Cell 93, 477.
      Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
      Varon, R., et al. 1998. Cell 93, 467.