PS1008 PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ch, H, M Rb Polyclonal Antibody

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PS1008-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
      Catalogue NumberPS1008
      Brand Family Calbiochem®
      SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
      Application Data
      Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
      References
      ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
      Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
      Preservative≤0.1 sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PS1008

      References

      Reference overview
      Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
      ApplicationImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application Data
      Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
      BackgroundFocal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that plays a key role in maintaining focal adhesion function and cell survival. It is phosphorylated following growth factor or integrin-mediated cell adhesion stimulation. FAK is localized to focal adhesions through its C-terminal focal adhesion targeting (FAT) domain that acts by binding to paxillin and talin that are associated with the cytoplasmic tails of integrins. Phosphorylation of FAK at Tyr861 by Src promotes this interaction between FAK and integrins.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
      IsotypeIgG
      Specieschicken, human, mouse
      Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
      Preservative≤0.1 sodium azide
      CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.