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324864 PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)

324864
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Overview

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Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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      Description
      OverviewRecognizes EGFR phosphorylated at Tyr1173 in vanadate-treated HepG2 cells.
      A positive control is provided.
      Catalogue Number324864
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹¹⁷³)
      Application Data
      Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate.
      Positive controlVanadate-treated HepG2 cells (included)
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/sample)
      Application CommentsDoes not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
      ImmunogenHuman
      Clone9H2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) Certificates of Analysis

      TitleLot Number
      324864
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-August-2007 RFH
      SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹¹⁷³)
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/sample)
      Application Data
      Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated on Tyr1173. Supplied with a positive control lysate derived from 2 x 106 vanadate-treated HepG2 cells.
      BackgroundBinding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues. The major site of tyrosine phosphorylation is tyrosine 1173.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
      Clone9H2
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlVanadate-treated HepG2 cells (included)
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsDoes not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
      Toxicity Standard Handling

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