324864
Sigma-AldrichPhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)
This PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of EGFR (pTyr¹¹⁷³).
More>>This PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of EGFR (pTyr¹¹⁷³). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Does not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
Immunogen
Human
Clone
9H2
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Documentation
PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) SDS
PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2) Certificates of Analysis
Title
Lot Number
324864
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.
Description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated on Tyr1173. Supplied with a positive control lysate derived from 2 x 106 vanadate-treated HepG2 cells.
Background
Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues. The major site of tyrosine phosphorylation is tyrosine 1173.
Host
Mouse
Immunogen species
Human
Immunogen
a synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
Clone
9H2
Isotype
IgG₁
Species
human, mouse, rat
Positive control
Vanadate-treated HepG2 cells (included)
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate.
Preservative
≤0.1% sodium azide (antibody only)
Comments
Does not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
