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69959 Perfect Protein™ Western Markers

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69959
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      Description
      Overview

      The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

      For ease of convenience, please consider the Perfect Protein AP Western Blot Kit (Cat. No. 69965), or the Perfect Protein HRP Western Blot Kit (Cat. No. 69078). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.


      A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
      B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.

      Catalogue Number69959
      Brand Family Novagen®
      Features and benefits• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
      • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
      • Can be used with colorimetric and chemiluminescent AP or HRP substrates.
      • Recombinant, unmodified markers give sharp, accurately sized bands.
      • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
      • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
      References
      Product Information
      FormLiquid
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Perfect Protein™ Western Markers SDS

      Title

      Safety Data Sheet (SDS) 

      Perfect Protein™ Western Markers Certificates of Analysis

      TitleLot Number
      69959

      Brochure

      Title
      Protein Purification and Detection Tools

      Citations

      Title
    • Athmane Teghanemt, et al. (2008) Novel roles in human MD-2 of phenylalanines 121 and 126 and tyrosine 131 in activation of toll-like receptor 4 by endotoxin. Journal of Biological Chemistry 283, 1257-1266.
    • Yulin Jia, et al. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. European Molecular Biology Organization Journal 19, 4004-4014.
    • User Protocols

      Title
      TB102 Perfect Protein™ Western Blot Kits

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      Categories

      Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents