70748 Pellet Paint® NF Co-Precipitant

Overview

Replacement Information

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70748-125UL
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      Glass bottle 125 ul
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      70748-3
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          Plastic ampoule 125 rxn
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          70748-4
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              Plastic ampoule 1000 rxn
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              70748-EA
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                  Glass bottle
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                  Description
                  Overview

                  Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrier per reaction and centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier. Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM® BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although Pellet Paint NF absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint NF) can be used as a correction factor when determining nucleic acid concentration. Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF Co-Precipitant is a useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used.

                  Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending

                  Catalogue Number70748
                  Brand Family Novagen®
                  Features and benefits
                  • Efficient and rapid precipitation of BigDye® cycle sequencing products
                  • Efficient removal of dye terminators
                  • Direct visualization and tracking of precipitated material
                  • No effect on sequencing reaction
                  • Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
                  References
                  Product Information
                  125 µl or 1 mlPellet Paint NF Co-Precipitant
                  1 ml or 8 ml3 M Sodium Acetate, pH 5.2
                  DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates.
                  Quality LevelMQ400
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
                  Toxicity Standard Handling
                  Storage -20°C
                  Avoid freeze/thaw Avoid freeze/thaw
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Pellet Paint® NF Co-Precipitant SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Pellet Paint® NF Co-Precipitant Certificates of Analysis

                  TitleLot Number
                  70748

                  Citations

                  Title
                • Taku Nakagawa, et al. (2007) Detection of circulating tumor cells in early-stage breast cancer metastasis to axillary lymph nodes. Clinical Cancer Research 13, 4105-4110.
                • Hikaru Taira, Takahiro Hohsaka and Masahiko Sisido. (2006) In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system. Nucleic Acids Research 34, 1653-1662.
                • Youn-Soo Lee, et al. (2005) Coexpression of erythropoietin and erythropoietin receptor in von Hippel-Lindau disease-associated renal cysts and renal cell carcinoma. Clinical Cancer Research 11, 1059-1064.
                • User Protocols

                  Title
                  TB268 Pellet Paint® NF Co-Precipitant

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                  Categories

                  Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up