Millipore Sigma Vibrant Logo

109254 Papanicolaou's solution 1b Hematoxylin solution S

for cytology

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109254
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
1092540500
Retrieving availability...
—
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic bottle 500 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      1092542500
      Retrieving availability...
      —
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic bottle 2.5 l
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          Catalogue Number109254
          DescriptionPapanicolaou’s solution 1b Hematoxylin solution S
          OverviewPapanicolaou’s solution 1b Hematoxylin solution S - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat no.109254) give a blue to dark violet staining result with clinical specimen material.
          A 500 ml bottle of either solution is sufficient for 1500 -2500 slides. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 00
          Quality LevelMQ400
          Applications
          Applicationfor cytology
          Biological Information
          Physicochemical Information
          Density1.05 g/cm3 (20 °C)
          pH value2.5 (H₂O, 20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Statement(s)H302: Harmful if swallowed.
          H319: Causes serious eye irritation.
          H373: May cause damage to organs through prolonged or repeated exposure if swallowed.
          Precautionary Statement(s)P260: Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
          P264: Wash skin thoroughly after handling.
          P270: Do not eat, drink or smoke when using this product.
          P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          P314: Get medical advice/ attention if you feel unwell.
          Signal WordWarning
          Storage class10 - 13 Other liquids and solids
          WGKWGK 1 slightly hazardous to water
          Disposal3
          Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
          Safety Information
          Categories of dangerharmful
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Suitability for microscopy (Vaginal smear)passes test
          Nucleiblue to dark violet
          Cyanophilic Cytoplasm (basophilic)blue-green
          Eosinophilic Cytoplasm (acidophilic)pink

          Related Products & Applications

          Accessories (Primary)

          Catalog Number Description  
          106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
          106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
          109253 Papanicolaou's solution 1a Harris' hematoxylin solution Show Pricing & Availability
          109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability
          115938 Hematoxylin monohydrate (C.I.75290) Show Pricing & Availability

          Accessories (Secondary)

          Catalog Number Description  
          104302 Hematoxylin cryst. (C.I. 75290) Show Pricing & Availability
          105175 Hematoxylin solution modified acc. to Gill II Show Pricing & Availability
          106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
          106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
          109253 Papanicolaou's solution 1a Harris' hematoxylin solution Show Pricing & Availability
          109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability

          Related Products By: Application Facete

          Clinical Cytology
          Cytodiagnosis
          Papanicolaou

          Categories

          IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents