539481 PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

539481
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539481-100KU
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      Description
      OverviewRecombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
      Catalogue Number539481
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      References
      ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
      Product Information
      Activity≥2.5 x 10⁶ U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
      EC number2.7.1.37
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsPhosphatases, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PKA, Catalytic Subunit, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PKA, Catalytic Subunit, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      539481

      References

      Reference overview
      Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

      Citations

      Title
    • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-January-2008 JSW
      SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      DescriptionRecombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
      Recommended reaction conditions50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 200 µM ATP, and γ-labeled-32P-ATP to a final specific activity of 200 μCi/μmol. Incubate at 30°C.
      EC number2.7.1.37
      ContaminantsPhosphatases, proteases: none detected
      Activity≥2.5 x 10⁶ U/ml
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
      Citation
    • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).