521270 PDK1, His•Tag®, Human, Recombinant, S. frugiperda

View Products on Sigmaaldrich.com
521270
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
521270-5UG
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic ampoule 5 μg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
      Catalogue Number521270
      Brand Family Calbiochem®
      Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
      References
      ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
      EC number2.7.10
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥75 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PDK1, His•Tag®, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      PDK1, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      521270

      References

      Reference overview
      Scott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-August-2014 JSW
      Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
      DescriptionFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      EC number2.7.10
      Purity≥90% by SDS-PAGE
      Specific activity≥75 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.