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521225 PDGF, BB Homodimer, Human, Recombinant, E. coli

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521225
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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521225-10UG
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      Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells.
      Catalogue Number521225
      Brand Family Calbiochem®
      SynonymsrhPDGF-BB
      References
      ReferencesAbedi, H., et al. 1998. FEBS Lett. 427, 209.
      Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.
      Product Information
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution, carrier free.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 ng/µg PDGF-BB
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PDGF, BB Homodimer, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PDGF, BB Homodimer, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      521225

      References

      Reference overview
      Abedi, H., et al. 1998. FEBS Lett. 427, 209.
      Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 JSW
      SynonymsrhPDGF-BB
      DescriptionRecombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal, and mononuclear cells. PDGF represents one of the major mitogens present in platelets. It exerts its effects by binding to specific high-affinity dimeric receptor tyrosine kinases. Material has the same amino acid sequence as mature human PDGF-B. Two distinct PDGF receptors have been identified: the PDGFα receptor, which binds to all three forms of PDGF, and the PDGFβ receptor, which can bind only PDGF-BB and PDGF-AB.
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution, carrier free.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 ng/µg PDGF-BB
      Biological activityED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells
      SolubilityReconstitute to a concentration of ≥100 µg/ml 100 mM acetic acid, 0.1% BSA. Further dilute with low endotoxin medium or buffered solution containing FBS or tissue culture grade BSA just prior to use.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 7525
      ReferencesAbedi, H., et al. 1998. FEBS Lett. 427, 209.
      Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors