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PCR Cleanup Filter Plates

Remove primers and unincorporated dNTPs in one step

Overview

Specifications

Ordering Information

Filter PlatesClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
LSKMPCR10MultiScreen PCRµ96 Filter Plate 10 Show Pricing & Availability
LSKMPCR50MultiScreen-PCRµ96 Filter Plate 50 Show Pricing & Availability
MSNU03010MultiScreen-PCR96 Filter Plate 10 Show Pricing & Availability
MSNU03050MultiScreen-PCR96 Filter Plate 50 Show Pricing & Availability
S384PCR10MultiScreen PCR384 Filter Plate 10 Show Pricing & Availability
S384PCR50MultiScreen PCR384 Filter Plate 50 Show Pricing & Availability

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Required EquimentClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz 1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz 1 Show Pricing & Availability
WP6110060Chemical Duty Pump, 100 V/50–60 Hz 1 Show Pricing & Availability

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Documentation

Data Sheet

Title
DNA Template Prep and Sequencing Reaction Cleanup
MultiScreen 384-PCR Filter Plate: Automation-friendly 384-well plate for high-throughput purification of PCR* products

Posters

Title
Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates
Poster: Purification Automation of Small Volume PCR Purification

References

Reference overviewApplication
PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations
Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D.
BioTechniques 29:844-857  2000

A Concise Guide to cDNA Microarray Analysis
P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush
BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000)  2000

PCR
A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs
Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309
J.Vet Diagn Invest 8:, 304-309  1996

Nucleic Acid Purification and Concentration
Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR
Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E.
BioTechniques. 19: 2, 282 – 285  1995

Nucleic Acid Purification and Concentration

FAQ

QuestionAnswer
Can the Montage PCRm96 plate be used in centrifugal mode?No. The Montage PCRm96 plate is designed for purification using vacuum filtration only.
Which Millipore manifold do I use with the Montage PCRm96 plate?The appropriate manifold is catalogue number SAVM38401. The unique micro-well design of the PCRm96 plate is not compatible with MultiScreen manifold catalogue number MAVM09601.
Will the Montage PCR96 and Montage PCRm96 plates remove primers and primer-dimers?The Montage PCR96 Cleanup Kit is designed to remove primers and other low molecular weight material from the PCR reaction. However, removal of primer dimers is problematic. The Montage PCRm96 plate removes > 99% of PCR primers, but although small primer-dimers are efficiently removed, quantitative removal of larger primer-dimers can be problematic given that the PCRm96 plate has been optimized for recovery of small PCR products. The best way to avoid complications due to primer-dimers is to optimize primer design.
When using Montage PCRm96 plates, do I need to do anything special to increase the recovery of my 100 - 300 bp PCR fragments?The protocol that was developed in conjunction with the PCRm96 plates has been optimized to provide maximal recovery of even small PCR products (e.g., 100 – 300 bp). No special modifications are necessary for small PCR fragments.
If I dilute my sample when using the Montage PCRu96 plate, will that increase the amount of time for filtration to be completed?Yes. Although sample dilution will marginally increase processing time, dilution is essential for maximal recovery of PCR products.
Is a wash step required following the initial filtration when using the Montage PCRu96 plate?The PCR product is sufficiently pure for DNA sequencing after a single vacuum filtration. If a particular application demands even higher purity, a wash step may be added.
What is the maximum vacuum that can be applied to the Montage PCRu96 plate?The recommended vacuum pressure for PCR purification is 20 inches of Hg. Exceeding this vacuum pressure will adversely affect recovery of PCR products and is not recommended.
Is the Montage PCRm96 plate capable of removing Triton or other detergents from the PCR reaction?No. The use of PCR reaction buffers that contain high concentrations of surfactants (i.e., greater than the critical micelle concentration) or protein stabilizers (e.g., gelatin) is not recommended for this application. Surfactants including Tween-20, Triton X-100 and Nonidet P-40 are not efficiently removed by the Montage PCRm96 plates and may result in carry-over into subsequent reactions.
Will the Montage PCRm96 remove mineral oil?The use of mineral oil is not recommended with the PCRm96 plate. Most thermocyclers use "Hot Bonnet" technology that eliminates the need for mineral oil.
What is the minimum recovery volume possible when using the Montage PCRm96 plates?Recovery of PCR fragments is possible using volumes as low as 20 mL.

User Guides

Title
Montage PCR%26lt;sub%26gt;96%26lt;/sub%26gt; Clean-Up Kit Laminated Card
Montage PCR%26lt;sub%26gt;96%26lt;/sub%26gt; Clean-Up Kit User Guide
MultiScreen 384 PCR Plate
MultiScreen PCR µ96 Plate