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71491 Overnight Express™ Instant TB Medium - Novagen

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              71491-60GM
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                  Description
                  Overview

                  Overnight Express™ Instant TB Medium is a granulated autoinduction culture medium for high-level protein production in pET and other IPTG-inducible bacterial expression systems. Instant TB Medium is a combination of Overnight Express Autoinduction System 1 components (Cat. No. 71300) with rich Terrific Broth (TB) medium and is supplied in two formats.
                  EasyPak is an aluminum foil pouch containing 60 g granulated medium, sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min to dissolve. Overnight Express Instant TB Medium is also available in 1 kg bottles. Granulated medium ensures rapid, uniform dissolution in water, prevents clumping of the medium, and avoids inhalation of airborne powder.

                  Overnight Express™ Autoinduction media are based on technology developed by F. William Studier at Brookhaven National Laboratory and enable regulated protein expression in E. coli,without monitoring the culture or adding IPTG during cell growth. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique autoinduction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems.

                  Cell mass and target protein yield are often increased several-fold compared to conventional protocols using induction with IPTG. Autoinduction media are extremely convenient for routine expression of proteins in multiple cultures and are ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones




                  EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min. Overnight Express Instant TB Medium is also available in 1 kg bottles.


                  In addition to Instant TB Medium, a variety of Overnight Express Autoinduction Media are available for different methods:

                  Overnight Express™ Autoinduction System 1 (71300) contains 3 sterile solutions, which can be added to your choice of glucose-free bacterial growth medium.

                  Overnight Express™ Autoinduction System 2 (71366) prepares a defined bacterial growth medium that can be used to selenomethionine-label proteins for x-ray crystallography.


                  Overnight Express Autoinduction NMR Media are 3 different media designed for cell growth Optimization (71760), 15N (71759) protein labeling, and 15N, 13C (71789) dual protein labeling for NMR analysis.
                  PRODUCT USE RESTRICTIONS - PRODUCT LABEL LICENSE

                  The Overnight Express™ Auto Induction Media material (Products) includes patented technology embodied therein developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy. The patent applications or issued patents embodied in this Product were assigned to Brookhaven Science Associates, LLC (BSA). BSA has enabled EMD Millipore and its Affiliates to grant a non-exclusive license for use of this patented technology, including use with the enclosed Products, based upon your acceptance of the following terms:
                  1. These Products are to be used for non-commercial research purposes only. For the avoidance of doubt, such non-commercial research purposes include research use by academic, non-profit, and commercial entities.
                  2. A separate license is required for any commercial use, including the use of the enclosed Products for developing or making other materials for sale to third parties.
                  3. Information about licenses for commercial use of the Products may be obtained from:

                  Office of Technology Commercialization and Partnerships
                  Brookhaven National Laboratory
                  Building No. 490C - P.O. Box 5000
                  Upton, New York 11973-5000
                  Telephone: 631-344-4711
                  Fax: 631-344-3729
                  E-mail: pupadhya@bnl.gov


                  4. Information about licenses for commercial manufacture and/or sale of the Products may be obtained from:

                  Intellectual Property Department
                  EMD Millipore Corporation
                  28820 Single Oak Drive
                  Temecula, CA 92590
                  E-mail: licensing@milliporesigma.com


                  5. No Products that contain this patented technology may be transferred or distributed further to third parties outside of your laboratory, unless the recipient third party receives a license from BSA and agrees to be bound by its terms.

                  By keeping or using the enclosed Product(s), you accept the above terms of use solely for noncommercial research purpose. If you refuse to accept the terms herein, you must return the Product(s) to EMD Millipore.
                  Catalogue Number71491
                  Brand Family Novagen®
                  References
                  Product Information
                  Quality LevelMQ200
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Standard Handling
                  Storage +15°C to +30°C
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Overnight Express™ Instant TB Medium - Novagen Certificates of Analysis

                  TitleLot Number
                  71491

                  Brochure

                  Title
                  Bulk packaging
                  Express yourself
                  Overnight Express Autoinduction Systems

                  Citations

                  Title
                • Erika J. Mancini, et al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 A resolution. Protein Science 16, 2294-2300.
                • Liguo Zhang, Kenneth P. Nephew and Patricia J. Gallagher. (2007) Regulation of death-associated protein kinase: stabilization by HSP90 heterocomplexes. Journal of Biological Chemistry 282, 11795-11804.
                • User Protocols

                  Title
                  TB526 Overnight Express™ Instant LB and TB Media

                  Related Products & Applications

                  Related Products

                  Catalog Number Description  
                  71300 Overnight Express™ Autoinduction System 1 - Novagen Show Pricing & Availability
                  71366 Overnight Express™ Autoinduction System 2 - Novagen Show Pricing & Availability

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                  Categories

                  Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Bacterial Culture Media