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509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

509290
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

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          Description
          OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
          Catalogue Number509290
          Brand Family Calbiochem®
          SynonymsNMD Inhibitor I
          References
          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
          Product Information
          CAS number108321-42-2
          FormWhite powder
          Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Quality LevelMQ200
          Applications
          Biological Information
          Biological activityAssay: ≥730 µg/mg
          Purity≥90% by HPLC
          Physicochemical Information
          ContaminantsHeavy metals: ≤100 ppm
          UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSCB9378500
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          509290

          References

          Reference overview
          Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-March-2020 JSW
          SynonymsNMD Inhibitor I
          DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
          FormWhite powder
          Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
          CAS number108321-42-2
          RTECSCB9378500
          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Purity≥90% by HPLC
          ContaminantsHeavy metals: ≤100 ppm
          UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
          Biological activityAssay: ≥730 µg/mg
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
          Toxicity Standard Handling
          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.