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71183 NucBuster™ Protein Extraction Kit

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      Glass bottle 100 rxns
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      OverviewThe NucBuster™ Protein Extraction Kit (Bruggink 2002) provides an alternative to the time intensive and cumbersome traditional methods for preparing nuclear extracts from mammalian cells. Time consuming traditional methods (up to 7 hours), based on a procedure originally described by Dignam et al. (1983), includes suspending cells in hypotonic solution, Dounce homogenization, centrifugation, and dialysis. The NucBuster Kit protocol is rapid and allows for the easy processing of multiple samples. The entire procedure from start to finish yields ready-to-use nuclear extract within 30 minutes. The composition of gentle detergents and salt in the final NucBuster extract is directly compatible with electrophoretic mobility shift assays (EMSA).

      The NucBuster protocol is based on two proprietary detergent-based solutions, NucBuster Extraction Reagent 1, optimized for cell lysis and removal of cytoplasmic components, and NucBusterExtraction Reagent 2, optimized for extraction of nuclear proteins. In addition, NucBuster extract is free of the 'stickiness' associated with release of genomic DNA, a problem associated with some traditional methods. No Dounce homogenization is required and there is no need for dialysis. The entire procedure is performed in a single microcentrifuge tube and requires only a vortex mixer and microcentrifuge. The kit provides enough reagents for 100 preparations of nuclear extract from 1 x 107 to 5 x 107 cells and the protocol is scalable.

      Catalogue Number71183
      Brand Family Novagen®
      Features and benefits
      • Nuclear extract preparation in 30 minutes
      • No homogenization or dialysis required
      • Procedure performed in a single microcentrifuge tube
      • Extracts suitable for activity assays, electrophoretic mobility shift assays (EMSA), and NoShift™ Transcription Factor Assays
      ReferencesBruggink, F. and Hayes, S. 2002. inNovations 15, 9.
      Dignam, J.D., et al. 1983. Nucl. Acids Res. 11, 1475.
      Product Information
      2 × 7.5 mlNucBuster Extraction Reagent 1
      7.5 mlNucBuster Extraction Reagent 2
      100 µl100 mM DTT
      1 setProtease Inhibitor Cocktail Set I (lyophilized, makes 100 µl)
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      71183-3 04055977254020


      NucBuster™ Protein Extraction Kit Certificates of Analysis

      TitleLot Number


      Reference overview
      Bruggink, F. and Hayes, S. 2002. inNovations 15, 9.
      Dignam, J.D., et al. 1983. Nucl. Acids Res. 11, 1475.


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