71091 NovaTaq™ Hot Start DNA Polymerase

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71091
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          Description
          OverviewNovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.



          Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
          Concentration 5.0 U/µl
          Endonuclease None detected
          Exonuclease None detected
          Amplification effiency Functional PCR
          Storage –20°C
          Catalogue Number71091 Brand Family Novagen®
          Features and benefits
          • Higher PCR specificity and yield
          • Improved low-copy target amplification
          • Ambient temperature setup compatible with automation
          • Target amplification of up to 5 kbp
          • Ideal for quantitative and high-throughput PCR applications
          References
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.
          250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)
          1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer
          1.5 ml or 5 × 1.5 ml25 mM MgCl₂
          DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
          Quality LevelMQ100
          Applications
          ApplicationNovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

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          Categories

          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > PCR & RT-PCR Kits & Reagents