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71377 NoShift™ Transcription Factor Assay Kit

An easy and rapid alternative to EMSA

NoShift™ Transcription Factor Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71377
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      		 Plastic ampoule 96 rxn
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      Description
      OverviewThe NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate.

      Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.
      Catalogue Number71377
      Brand Family Novagen®
      References
      ReferencesFried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.
      Product Information
      •4 × 1 ml4X NoShift™ Bind Buffer
      •150 µlSalmon Sperm DNA
      •125 µlPoly(dI:dC):Poly(dI:dC)
      •30 ml10X NoShift Wash Buffer
      •20 mlNoShift Antibody Dilution Buffer
      •1 eaStreptavidin Plate
      •12 mlTMB Substrate
      •100 µl100 mM DTT
      •3 eaAluminum Plate Sealer
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Identification Number
      Catalog Number GTIN
      71377-34055977270570

      Documentation

      NoShift™ Transcription Factor Assay Kit Certificates of Analysis

      TitleLot Number
      71377

      References

      Reference overview
      Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.

      Citations

      Title
    • Markus Bredel, et al. (2006) Tumor necrosis factor-α-induced protein 3 as a putative regulator of nuclear factor-κB-mediated resistance to O6-alkylating agents in human glioblastomas. Journal of Clinical Oncology 24, 274-287.
    • Zachary T. Resch, et al. (2005) Stress-activated signaling pathways mediate the stimulation of pregnancy-associated plasma protein-A expression in cultured human fibroblasts. Endocrinology 147, 885-890.
    • Andrei P. Pomerantsev, Olga M. Pomerantsev and Stephen H. Leppla. (2004) A spontaneous translational fusion of Bacillus cereus plcR and papR activates transcription of plcR-dependent genes in Bacillus anthracis via binding with a specific pallindromic sequence. Infection and Immunity 72, 5814-5823.
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      User Protocols

      Title
      TB403 NoShift™ Transcription Factor Assay Kit