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71377 NoShift™ Transcription Factor Assay Kit

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      OverviewThe NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate.

      Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.

      Catalogue Number71377
      Brand Family Novagen®
      ReferencesFried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.
      Product Information
      4 × 1 ml4X NoShift™ Bind Buffer
      150 µlSalmon Sperm DNA
      125 µlPoly(dI:dC):Poly(dI:dC)
      30 ml10X NoShift Wash Buffer
      20 mlNoShift Antibody Dilution Buffer
      1 eaStreptavidin Plate
      12 mlTMB Substrate
      100 µl100 mM DTT
      3 eaAluminum Plate Sealer
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Identification Number
      Catalog Number GTIN


      NoShift™ Transcription Factor Assay Kit Certificates of Analysis

      TitleLot Number


      Reference overview
      Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.


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    • User Protocols

      TB403 NoShift™ Transcription Factor Assay Kit