482777 Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli

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482777
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482777-50U
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      Description
      OverviewRecombinant, mouse inducible NOS expression in E. coli.
      Catalogue Number482777
      Brand Family Calbiochem®
      SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
      References
      ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
      White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
      Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
      FormLiquid
      FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥2.9 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20/21/22-36/37/38

      Harmful by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      482777

      References

      Reference overview
      Stevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
      White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
      Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-January-2011 JSW
      SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
      DescriptionRecombinant, mouse inducible NOS expression in E. coli.
      FormLiquid
      FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions50 mM HEPES, 1 mM arginine, 1 mM magnesium acetate, 0.1 mM NADPH, 5 µM oxyhemoglobin, 12 µM tetrahydrobiopterin, 170 µM DTT, pH 7.4.
      Specific activity≥2.9 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Harmful
      ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
      White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
      Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.