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362300 N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

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362300
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      Description
      OverviewRecombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides.
      Note: 1 mU = 1 milliunit.
      Catalogue Number362300
      Brand Family Calbiochem®
      SynonymsPeptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F
      References
      ReferencesFan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
      Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
      Product Information
      CAS number83534-39-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.
      EC number3.5.1.52
      FormLiquid
      FormulationEnzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥10 units/mg protein
      Physicochemical Information
      Contaminantsβ-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      362300

      References

      Reference overview
      Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
      Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-March-2019 JSW
      SynonymsPeptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F
      DescriptionRecombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.
      FormLiquid
      FormulationEnzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
      Recommended reaction conditions

      • Reaction Buffer (Component No. KP31859-1EA): 1 vial, 1 ml, 100 mM sodium phosphate, 0.1% sodium azide, pH 7.5, supplied as a 5X solution
      • Denaturation Solution (Component No. KP31860-1EA): 1 vial, 200 µl, 2% SDS, 1 M β-mercaptoethanol
      • Detergent Solution (Component No. KP31861-1EA): 1 vial, 200 µl, 15% NP-40 solution
      • Tris Reaction Buffer (Component No. KP31865-1EA): 1 vial, 1 ml, 50 mM Tris-HCl, pH 8.0
      • N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli (Component No. KP31858-100MIU): 1 vial, 100 mU
      Note: Tris Reaction Buffer has been included as an alternative reaction buffer because phosphate buffers should be avoided if mass spectrometry is used in downstream analysis.

      Procedure for Deglycosylation (Denaturing Conditions)
      The amount of enzyme required for deglycosylation depends on the substrate used, the incubation conditions, and the exact application. This protocol is provided only as a guide. Assay conditions should be adjusted as required for individual applications.
      1. Prepare 50 to 500 µg glycoprotein solution in 45 µl 1X Reaction. Add 2.5 µl Denaturation Solution.
      2. Denature glycoprotein by heating at 100°C for 5 min. Allow mixture to cool.
      3. Add 2.5 µl Detergent Solution.
      4. Add 2 µl N-Glycosidase to the reaction mixture and incubate for 2 h to overnight at 37°C.
      CAS number83534-39-8
      EC number3.5.1.52
      Contaminantsβ-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases: none detected
      Specific activity≥10 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
      Altmann, F., et al. 1995. Glycoconj. J. 12, 84.