411483 Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

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411483
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411483-200UG
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      Plastic ampoule 200 μg
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      Description
      Catalogue Number411483
      Brand Family Calbiochem®
      References
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA, pH 7.4.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Application NotesELISA (1:500-1:2000, see comments)
      Application CommentsBiotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: ≤0.1%
      Human IgG2: ≤1%
      Human IgG3: 100%
      Human IgG4: ≤0.1%
      Human IgA: ≤0.1%
      Human IgE: ≤0.1%
      Human IgM: ≤0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.001%
      Human IgG2: <0.001%
      Human IgG3: 100%
      Human IgG4: <0.001%
      Human IgA: <0.001%
      Human IgE: <0.001%
      Human IgM: <0.001%
      Biological Information
      ImmunogenHuman
      CloneHP6047
      HostMouse
      IsotypeIgG₃
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate Certificates of Analysis

      TitleLot Number
      411483

      References

      Reference overview
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      ApplicationELISA (1:500-1:2000, see comments)
      DescriptionPurified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region.
      HostMouse
      Immunogen speciesHuman
      CloneHP6047
      IsotypeIgG₃
      FormLiquid
      FormulationIn PBS, 1% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsBiotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: ≤0.1%
      Human IgG2: ≤1%
      Human IgG3: 100%
      Human IgG4: ≤0.1%
      Human IgA: ≤0.1%
      Human IgE: ≤0.1%
      Human IgM: ≤0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.001%
      Human IgG2: <0.001%
      Human IgG3: 100%
      Human IgG4: <0.001%
      Human IgA: <0.001%
      Human IgE: <0.001%
      Human IgM: <0.001%
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.