Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
What is the blue separation paper between my filters?
It is a parchmentalized paper specially formulated for Millipore Corporation.
How do I tell the membrane from the separator?
The blue paper is the separator.
Can Millipore filters remove mycoplasma from my solution?
Mycoplasma can be removed from solutions by using a 0.1 um pore size filter. For mycoplasma reduction the 0.1 um Durapore filter can be used. Its Millipore code is VV. A 0.1 um Express membrane can also be used for removal of mycoplasma. Its membrane code is VP. These membranes are available in a number of fabricated fiter devices. For help determining which device would be best for your solution, call Millipore Technical Services.
Which membrane should I use to filter my solution?
For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
Which side of the membrane should I use, the shiny or dull side?
Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
What is the difference between pore size and pore size distribution?
Whereas pore size is a measure of the diameter of the largest pore, pore size distribution is a measure of the range of pore sizes. The range of pore sizes can be normally distributed, and the spread can be quite narrow (e.g. the ratio of largest to smallest may be less than 2). On the other hand, pore size distribution can be very heterogeneous. In the case of large spreads and heterogeneity, the pore size will be far less predictive of flow rate (either filtration or capillary) than it will be for a membrane with a narrow pore size distribution. It is important to note that the pore size corresponding to the bubble point is not at the middle of the distribution, but is the largest pore.
Is there a sidedness to the Express PES membrane?
Yes, this membrane should be oriented shiny side down.
Is the Express membrane surfactant free?
Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
The Millipore Express® PLUS membrane provides ultrafast filtration of tissue culture media, additives, buffers and other aqueous solutions. This high throughput, low protein-binding membrane is also used in many of our ready-to-use sterile filtration devices.
Features & Benefits
Ultra fast filtration and high throughput shorten process time
Can be sterilized by autoclave, ethylene oxide, or gamma irradiation for sterile filtration applications
Applications
Sterile Filtration, Buffer Filtration, Tissue Culture Media Filtration
Product Performance
Color: white Surface: plain Thickness: ≥150 µm and ≤200 µm Sterilization: by autoclave (121 °C at 1 bar), EO or gamma Bacterial endotoxins: 0.5 EU/mL Gravimetric extractables: <1.5% Flow rate: 40 mL/min cm2 at 2 bar, 25 °C
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