ECM101 Millicoat™ Human Fibronectin Coated Strips (96-Wells)

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ECM101
1 plate  96 wells
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsDetection Methods
      ACT Chromogenic
      Description
      Catalogue NumberECM101
      Brand Family Chemicon®
      Trade Name
      • Millicoat
      • Chemicon
      DescriptionMillicoat™ Human Fibronectin Coated Strips (96-Wells)
      OverviewCell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with Human Fibronectin. Row H of each strip is coated with BSA which serves as a negative assay control. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
      Alternate Names
      • Formerly
        under the CytoMatrixTM brand name.
      References
      Product Information
      Detection methodChromogenic
      Quality LevelMQ100
      Applications
      ApplicationMillicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a 96-well plate frame for convenience & flexibility in designing assays.
      Key Applications
      • Activity Assay
      Application NotesPROCEDURE:

      NOTE: Optimal assay timing and performance may vary for different cell lines but generally can be obtained using subconfluent cell cultures in the assay described below. Subconfluent cultures can be achieved by splitting cells 1 to 2 days prior to performing the assay.

      1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydrated strips.

      2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.

      3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 2-3 times with PBS containing Ca2+/Mg2+ (200 mL/well).

      4. Add 100 mL of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).

      5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO4, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.

      6. Determine the absorbance at 540 - 570 nm on a microplate reader.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMay be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Millicoat™ Human Fibronectin Coated Strips (96-Wells) SDS

      Title

      Safety Data Sheet (SDS) 

      Millicoat™ Human Fibronectin Coated Strips (96-Wells) Certificates of Analysis

      TitleLot Number
      Millicoat CELL ADHESION STRIPS HUMAN FIBRONECTIN -
      MillicoatTM CELL ADHESION STRIPS -26989102698910
      MillicoatTM CELL ADHESION STRIPS HUMAN FIBRONECTIN2825543
      MillicoatTM CELL ADHESION STRIPS HUMAN FIBRONECTIN - 30615403061540

      References

      Reference overviewPub Med ID
      Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs.
      A Blitek,E Morawska,A J Ziecik
      Theriogenology  78  2012

      Show Abstract
      22920593 22920593
      Interactions between galectin-3 and integrinbeta3 in regulating endometrial cell proliferation and adhesion.
      Lei CX, Zhang W, Zhou JP, Liu YK
      Human reproduction (Oxford, England)  24  2879-89  2009

      Show Abstract
      19633306 19633306

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Adhesion Assays