ECM205 Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105

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      Replacement Information

      Key Specifications Table

      Key ApplicationsDetection Methods
      ACT Chromogenic
      Catalogue NumberECM205
      Brand Family Chemicon®
      Trade Name
      • Millicoat
      • Chemicon
      DescriptionMillicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
      OverviewCell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
      Alternate Names
      • Formerly under the CytoMatrix brand name.
      Product Information
      Detection methodChromogenic
      Quality LevelMQ100
      ApplicationThe MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.
      Key Applications
      • Activity Assay
      Application NotesPROCEDURE:

      NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.

      1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.

      2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.

      3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).

      4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).

      5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.

      6. Determine the absorbances at 540 - 570 nm on a microplate reader.
      Biological Information
      Species Reactivity
      • Human
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMay be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
      Packaging Information
      Material Size1 set
      Transport Information
      Supplemental Information


      Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105 Certificates of Analysis

      TitleLot Number
      CytoMatrixTM (5) SCREENING KIT (1 each of ECM101 - ECM105) -
      MillicoatTM SCREENING KIT (1 each of ECM101 - ECM105)3050192
      MillicoatTM SCREENING KIT (1 each of ECM101 - ECM105)2885322
      MillicoatTM SCREENING KIT (1 each of ECM101 - ECM105)3110198
      MillicoatTM SCREENING KIT (1 each of ECM101 - ECM105)2950041
      MillicoatTM SCREENING KIT - 34783233478323
      MillicoatTM SCREENING KIT -27337762733776


      Reference overviewPub Med ID
      Calreticulin has opposing effects on the migration of human trophoblast and myometrial endothelial cells.
      K E Crawford,B Kalionis,J L Stevenson,S P Brennecke,N M Gude
      Placenta  33  2012

      Show Abstract
      22377355 22377355
      Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis.
      Tomonori Kaneda,Yoshiko Sonoda,Kumi Ando,Takaharu Suzuki,Yasuhiro Sasaki,Tomoyuki Oshio,Megumi Tago,Tadashi Kasahara
      Cancer letters  270  2008

      Show Abstract
      18606490 18606490

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