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PICM03050 Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

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PICM03050
50  Individually blister packaged
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      Overview

      Replacement Information
      Description
      Catalogue NumberPICM03050
      Trade Name
      • Millicell
      DescriptionMillicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm
      Background InformationWith Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

      Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains.

      Insert Formats:
      Millicell Hanging Inserts: Preloaded in receivers or available standalone
      - For co-culturing and permeability assays
      - Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
      - Available in 5 pore sizes and 3 diameters, including a 1 µm pore size that is optically transparent for better visualization by microscopy
      - NEW: Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters


      Millicell Standing Inserts:
      - Promotes excellent cell growth and provides an exceptional opportunity for cell studies
      - Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane

      Millicell Organotypic Insert:
      - For high cell viability and superior study of three dimensional explant structure
      - Lower height allows them to fit inside a standard petri dish
      - The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
      - The membrane is optically clear and optimized for long-term organotypic explant maintenance

      Membrane Types:
      Biopore Membrane (hydrophilic PTFE)
      - For low protein-binding, live cell viewing, and immunofluorescent applications

      MF-Millipore™ Membrane (mixed cellulose esters)
      - For exceptional anatomical and functional polarization

      Isopore™ Membrane (polycarbonate)
      - For growth of attachment-dependent cells without matrix

      PET Membrane (polyethylene terephthalate)
      - For growth of attachment-dependent cells without matrix

      With Millicell® inserts, adherent or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

      Applications

      Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry
      References
      Product Information
      Device Configuration6-well plate
      Filter CodeCM
      Maximum Operating Temperature50 °C
      Number of Wells1
      Quality LevelMQ400
      Applications
      ApplicationHydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
      Key Applications
      • Cell Attachment
      • Cell Culture
      • Cell Differentiation
      • Fluorescence
      • Low Protein Binding
      Biological Information
      MediaBiopore®
      SterilitySterile
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Pore Size0.4 µm
      Dimensions
      Height13 mm
      Diameter3.15 cm
      Filtration Area4.2 cm²
      Filter Diameter (⌀)30 mm
      Materials Information
      Chemistry
      • Hydrophilic Polytetrafluoroethylene (PTFE)
      Device Material
      • Polystyrene
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size50
      Material PackageIndividually blister packaged
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
      Landucci, Elisa, et al.
      Neuropharmacology, 60: 674-82 (2011)  2011

      Show Abstract
      21130785 21130785
      Meiosis initiation in the human ovary requires intrinsic retinoic acid synthesis.
      Le Bouffant, R, et al.
      Hum. Reprod., 25: 2579-90 (2010)  2010

      Show Abstract
      20670969 20670969
      Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
      Letellier, Mathieu, et al.
      Proc. Natl. Acad. Sci. U.S.A., 106: 14102-7 (2009)  2009

      Show Abstract
      19666592 19666592
      Preparation of organotypic hippocampal slice cultures: interface method.
      De Simoni, Anna and Yu, Lily M Y
      Nat Protoc, 1: 1439-45 (2006)  2006

      Show Abstract
      17406432 17406432
      Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination.
      Cupp, Andrea S, et al.
      Biol. Reprod., 68: 2033-7 (2003)  2003

      Show Abstract
      12606390 12606390
      The pro-apoptotic gene Bax is required for the death of ectopic primordial germ cells during their migration in the mouse embryo.
      Stallock, James, et al.
      Development, 130: 6589-97 (2003)  2003

      Show Abstract
      14660547 14660547
      A reliable method for organ culture of neonatal mouse retina with long-term survival.
      Ogilvie, J M, et al.
      J. Neurosci. Methods, 87: 57-65 (1999)  1999

      Show Abstract
      10065994 10065994
      Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
      Spooner, B S, et al.
      J. Exp. Zool., 269: 212-22 (1994)  1994

      Show Abstract
      8014615 8014615
      Growth and morphogenesis of embryonic mouse organs on non-coated and extracellular matrix-coated Biopore membrane.
      Hardman, P, et al.
      Dev. Growth Differ., 35: 683-90 (1993)  1993

      Show Abstract
      11538317 11538317

      User Guides

      Title
      MILLICELL STANDING INSERT

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