SCR089 MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cell Kit

SCR089
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      Overview

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      Cult
      Description
      Catalogue NumberSCR089
      Trade Name
      • MilliTrace
      • Chemicon
      DescriptionMilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cell Kit
      OverviewMilliTraceTM Nanog GFP Reporter Mouse Embryonic Stem Cell Kit (Catalog No. SCR089) provides ready-to-use mouse embryonic stem cells that express the humanized mulleri green fluorescent protein (hmGFP) reporter gene under the control of the mouse Nanog promoter along with expansion medium to help maintain expression of the transgene.

      MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cells were generated by transfection of C57/BL6 mouse embryonic stem cells with a proprietary bicistronic plasmid construct containing hmGFP under the control of the mouse Nanog promoter. FACS analyses of stable transfectants indicate that greater than 90% of the cells express GFP at high levels even after 10 passages when cultured at normal proliferation conditions. MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cells display the immunochemical staining properties of pluripotent stem cells; they are positive for alkaline phosphatase and Nanog-hmGFP expression is co-localized with Oct-4, Sox-2, and SSEA-1 expression. Upon differentiation, hmGFP is down-regulated and cells have the capacity to differentiate into multiple lineages, including cardiomyocytes and neurons. Under neuronal differentiation conditions, cells can be differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+) (Use Mouse Embryonic Stem Cell Neurogenesis Kit, Catalog No. SCR101).
      Background InformationNanog is an important marker of undifferentiated embryonic stem cells and, along with Oct-4 and Sox-2, is an important regulator of stem cell pluripotency. Studies have shown that down-regulation of the Nanog promoter coincides with the loss of pluripotency and progression towards a differentiated state. Nanog GFP Reporter Mouse Embryonic Stem Cell Kit provides a quick non-invasive method by which to monitor the expression of Nanog in pluripotent embryonic stem cells. The cells can be used to facilitate studies elucidating the role of Nanog and other factors in the maintenance and self-renewal of embryonic stem cells. This kit can also be used to identify factors involved in embryonic stem cell differentiation.
      References
      Product Information
      Components
      • MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cells, 1 x 106 viable cells
      • MilliTrace Mouse Embryonic Stem Cell Expansion Medium (Catalog No. SCM042)
      Applications
      ApplicationMilliTraceTM Nanog GFP Reporter Mouse Embryonic Stem Cell Kit.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      Cell Line Type
      • Embryonic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceNanog GFP reporter mouse embryonic stem cells have been validated for expression of hmGFP in the pluripotent state, for high level of expression of alkaline phosphatase, Oct-4, Sox-2, and SSEA-1, and for their self-renewal and multi-lineage differentiation capacities. Approximately 80% cells display normal karyotype as assessed by G-banding of twenty metaphase cells. Cells tested negative for mycoplasma.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
      Storage and Shipping Information
      Storage Conditions· MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cells (Part No. SCC089) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
      · EmbryoMax Complete ES Cell Medium with 15% FBS and LIF (Part No. ES-101-B) should be stored at -20°C until expiration date on the label. Upon thawing the medium should be stored at 2-8°C and given a 1-month expiration dating.
      · 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MilliTrace Nanog GFP Reporter Mouse Embryonic Stem Cell Kit SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells.
      Leslie A Kirby,Jason T Schott,Brenda L Noble,Daniel C Mendez,Paul S Caseley,Sarah C Peterson,Tyler J Routledge,Nilay V Patel
      PloS one  7  2012

      Show Abstract
      22745733 22745733

      Technical Info

      Title
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling (EMD)

      User Guides

      Title
      MilliTrace™ Nanog GFP Reporter Mouse Embryonic Stem Cell Kit

      Related Products & Applications

      Accessories

      Catalog Number Description  
      SCM042 MilliTrace Mouse Embryonic Stem Cell Expansion Medium Show Pricing & Availability
      SCR101 Mouse Embryonic Stem Cell Neurogenesis Kit Show Pricing & Availability

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines