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FCMAB252F Milli-Mark™ Anti-GAPDH-FITC Antibody, clone 6C5

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FCMAB252F
100 tests  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB252F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-GAPDH-FITC Antibody, clone 6C5
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase, GAPDH
      • G3PDH
      • GAPD
      • OK/SW-cl.12
      • MGC88685
      • G3PD
      • EC 1.2.1.12
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatFITC
      Control
      • A431 cells
      PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationMilli-Mark Anti-GAPDH-FITC Antibody, clone 6C5 is a purified monoclonal antibody labeled with Fluorescein isothiocyanate (FITC). This well characterized mAb specifically detects Glyceraldehyde-3-Phosphate Dehydrogenase and is validated for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenGlyceraldehyde-3-phosphate dehydrogenase purified from rabbit muscle
      Clone6C5
      HostMouse
      SpecificityAntibody recognizes Human Glyceraldehyde-3-Phosphate Dehydrogenase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

      SIZE: 335 amino acids; 36053 Da

      SUBUNIT: Homotetramer. Binds PRKCI.

      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

      SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight36 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Milli-Mark™ Anti-GAPDH-FITC Antibody, clone 6C5 SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark™ Anti-GAPDH-FITC Antibody, clone 6C5 Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-GAPDH-FITC, clone 6C5 - 2151864 2151864
      Milli-Mark Anti-GAPDH-FITC, clone 6C5 - 2207225 2207225
      Milli-Mark Anti-GAPDH-FITC, clone 6C5 - NRG1830303 NRG1830303
      Milli-Mark™ Anti-GAPDH-FITC, -2550906 2550906
      Milli-Mark™ Anti-GAPDH-FITC, -2639393 2639393
      Milli-Mark™ Anti-GAPDH-FITC, clone 6C5 2471887
      Milli-Mark™ Anti-GAPDH-FITC, clone 6C5 -2480871 2480871
      Milli-Mark™ Anti-GAPDH-FITC, clone 6C5 -2700959 2700959
      Milli-Mark™ Anti-GAPDH-FITC, clone 6C5 - 2375593 2375593
      Milli-Mark™ Anti-GAPDH-FITC, clone 6C5 - 2283891 2283891

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies