475858 Met30 Antagonist, SMER3 - Calbiochem

475858
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₁H₄N₄O₂

Pricing & Availability

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475858-25MG
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      Glass bottle 25 mg
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      Description
      OverviewA cell-permeable indenofurazinopyrazinone compound that is shown to directly target the F-box protein Met30 subunit of the Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex, disrupting Met30-Skp1 interaction and selectively inhibiting SCFMet30, but not SCFCdc4, E3 ubiquitin ligase activity. SMER3 is reported to act in a synthetic lethal fashion with rapamycin (Cat. Nos. 553210, 553211, & 553212) in both human lung cancer A549 and in yeast cultures. SMER3 treatment (10 to 30 µM) results in cell cycle arrest and morphological phenotype resembling that seen with temperature-sensitive Met30, but not Skp1 or Cdc4, mutant under non-permissive temperature in yeast cultures.
      Catalogue Number475858
      Brand Family Calbiochem®
      SynonymsSmall-Molecule Enhancer of Rapamycin-3, 9H-Indeno[1,2-b][1,2,5]oxadiazolo[3,4-e]pyrazin-9-one, Indeno[1,2-b]furazano[3,4-E]pyrazin-9-one, SCFMet30 Inhibitor
      References
      ReferencesAghajan, M., et al. 2010. Nature Biotechnol. 28, 738.
      Product Information
      FormYellow solid
      Hill FormulaC₁₁H₄N₄O₂
      Chemical formulaC₁₁H₄N₄O₂
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Regulatory Review
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Met30 Antagonist, SMER3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Met30 Antagonist, SMER3 - Calbiochem Certificates of Analysis

      TitleLot Number
      475858

      References

      Reference overview
      Aghajan, M., et al. 2010. Nature Biotechnol. 28, 738.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-October-2011 RFH
      SynonymsSmall-Molecule Enhancer of Rapamycin-3, 9H-Indeno[1,2-b][1,2,5]oxadiazolo[3,4-e]pyrazin-9-one, Indeno[1,2-b]furazano[3,4-E]pyrazin-9-one, SCFMet30 Inhibitor
      DescriptionA cell-permeable indenofurazinopyrazinone compound that is shown to directly target the F-box protein Met30 subunit of the Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex, disrupting Met30-Skp1 interaction and selectively inhibiting SCFMet30, but not SCFCdc4, E3 ubiquitin ligase activity. SMER3 is reported to act in a synthetic lethal fashion with rapamycin (Cat. Nos. 553210, 553211, & 553212) in both human lung cancer A549 and in yeast cultures. SMER3 treatment (10 to 30 µM) results in cell cycle arrest and morphological phenotype resembling that seen with temperature-sensitive Met30, but not Skp1 or Cdc4, mutant under non-permissive temperature in yeast cultures.
      FormYellow solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₁H₄N₄O₂
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (10 mg/ml). It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Regulatory Review
      ReferencesAghajan, M., et al. 2010. Nature Biotechnol. 28, 738.