Millipore Sigma Vibrant Logo

17-700 Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

View Products on Sigmaaldrich.com
17-700
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      RIP
      Description
      Catalogue Number17-700
      Brand Family Upstate
      Trade Name
      • Magna RIP
      • Upstate
      DescriptionMagna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Negative controls
      Alternate Names
      • Magnetic RIP kit
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      References
      Product Information
      Components
      • Magnetic Beads Protein A/G
      • RIP Wash Buffer
      • RIP Lysis Buffer
      • 0.5 M EDTA
      • 10% SDS
      • Salt Solution I
      • Salt Solution II
      • Precipitate Enhancer
      • Normal Mouse IgG
      • Rabbit IgG Purified
      • Protease Inhibitor Cocktail 200X
      • RNase Inhibitor
      • Proteinase K (10 mg/mL)
      • Nuclease free water
      PresentationTwo boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Quality LevelMQ100
      Applications
      ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size12 assays
      Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      17-700 04053252010880

      Documentation

      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit Certificates of Analysis

      TitleLot Number
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit - 3678915 3678915

      References

      Reference overviewApplicationPub Med ID
      LncRNA-PVT1 promotes pancreatic cancer cells proliferation and migration through acting as a molecular sponge to regulate miR-448.
      Zhao, L; Kong, H; Sun, H; Chen, Z; Chen, B; Zhou, M
      J Cell Physiol  233  4044-4055  2018

      Show Abstract
      28657147 28657147
      LncRNA ZFAS1 promotes cell migration and invasion of fibroblast-like synoviocytes by suppression of miR-27a in rheumatoid arthritis.
      Ye, Y; Gao, X; Yang, N
      Hum Cell  31  14-21  2018

      Show Abstract
      28721682 28721682
      Effects of long noncoding RNA SPRY4-IT1-mediated EZH2 on the invasion and migration of lung adenocarcinoma.
      Wen, X; Han, XR; Wang, YJ; Fan, SH; Zhuang, J; Zhang, ZF; Shan, Q; Li, MQ; Hu, B; Sun, CH; Wu, Q; Tan, JH; Wu, DM; Lu, J; Zheng, YL
      J Cell Biochem  119  1827-1840  2018

      Show Abstract
      28796375 28796375
      MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression.
      Liu, L; Luo, C; Luo, Y; Chen, L; Liu, Y; Wang, Y; Han, J; Zhang, Y; Wei, N; Xie, Z; Wu, W; Wu, G; Feng, Y
      Oncogene  37  86-94  2018

      Show Abstract
      28869607 28869607
      RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis.
      Sutherland, JM; Sobinoff, AP; Fraser, BA; Redgrove, KA; Siddall, NA; Koopman, P; Hime, GR; McLaughlin, EA
      J Cell Physiol  233  3262-3273  2018

      Show Abstract
      28884822 28884822
      Long non-coding RNA MALAT1 promotes oral squamous cell carcinoma development via microRNA-125b/STAT3 axis.
      Chang, SM; Hu, WW
      J Cell Physiol  233  3384-3396  2018

      Show Abstract
      28926115 28926115
      Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma.
      Zhao, X; Liu, Y; Li, Z; Zheng, S; Wang, Z; Li, W; Bi, Z; Li, L; Jiang, Y; Luo, Y; Lin, Q; Fu, Z; Rufu, C
      J Cell Mol Med  22  655-667  2018

      Show Abstract
      28984028 28984028
      Functional Role of a Novel Long Noncoding RNA TTN-AS1 in Esophageal Squamous Cell Carcinoma Progression and Metastasis.
      Lin, C; Zhang, S; Wang, Y; Wang, Y; Nice, E; Guo, C; Zhang, E; Yu, L; Li, M; Liu, C; Hu, L; Hao, J; Qi, W; Xu, H
      Clin Cancer Res  24  486-498  2018

      Show Abstract
      29101304 29101304
      Lnc-NTF3-5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR-93-3p.
      Peng, W; Zhu, SX; Wang, J; Chen, LL; Weng, JQ; Chen, SL
      Clin Implant Dent Relat Res  20  110-121  2018

      Show Abstract
      29106055 29106055
      Long non-coding RNA GAS5 antagonizes the chemoresistance of pancreatic cancer cells through down-regulation of miR-181c-5p.
      Gao, ZQ; Wang, JF; Chen, DH; Ma, XS; Yang, W; Zhe, T; Dang, XW
      Biomed Pharmacother  97  809-817  2018

      Show Abstract
      29112934 29112934

      Related Products & Applications

      Accessories

      Catalog Number Description  
      20-400 Magna GrIP™ Rack (8 well) Show Pricing & Availability
      CDUFBC001 BioPak® C Clinical Cartridge Show Pricing & Availability
      CDUFBI001 Biopak® Polisher Show Pricing & Availability
      MBPKMNKIT BioPak® C Wall Mounting Bracket Show Pricing & Availability

      Related Products By: Brand Facete

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits