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17-10499 Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine

10 Assays  
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      Key Specifications Table

      Key Applications
      RNA Detection, Purification with magnetic beads, RIP
      Catalogue Number17-10499
      Trade Name
      • EZ-Magna RIP
      DescriptionMagna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine
      OverviewN6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes. The mark is deposited by a heterodimer of methyltransferase-like 3 and 14 (Mettl3 and Mettl14) and can be removed by the RNA demethylase enzymes FTO and ALKBH5. Obesity risk gene FTO encodes the first identified m6A demethylase. Mutations in FTO have been associated with increased risk for obesity and type II diabetes. Recently, impairment of the status of m6A regulation has lead to prolonged expression of Nanog in ES cells and the inability to exit self-renewal stages toward differentiation.

      Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios.

      Features and Benefits

      • Complete collection of MeRIP valiidated reagents to streamline the MeRIP method.
      • High signal to noise ratios using high affinity m6A monoclonal antibody and A/G magnetic beads.
      • Stem cell validated.
      Alternate Names
      • RNA Methylation Kit
      • MeRIP-Seq Kit
      • N6-Methyladenosine Kit
      • M6A Immunoprecipitation Kit
      Product Information
      • RNA Fragmentation Buffer 10X (0.5 mL)
      • 0.5M EDTA (0.5 mL)
      • IP Buffer 5X (15 mL)
      • Magnetic Beads A/G Blend (315 µL)
      • Anti-N6-methyladenosine (m6A), clone 17-3-4-1 (100 µg)
      • RNase Inhibitor (90 µL)
      • N6-Methyladenosine, 5′-monophosphate sodium salt (m6A) (10 mg)
      • Normal Mouse IgG (125 µg)
      • MeRIP Primers Human EEF1A1 Positive (75 µL)
      • MeRIP Primers Human EEF1A1 Negative (75 µL)
      Quality LevelMQ300
      ApplicationThe Magna MeRIP™ m6A Kit streamlines the MeRIP (Methylated RNA Immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A (N6-methyladenosine) RNA methylation sites. Suitable for MeRIP-Seq and qRT-PCR of methylated RNA transcripts.
      Key Applications
      • RNA Detection
      • Purification with magnetic beads
      • RNA Binding Protein Immunoprecipitation (RIP)
      Application NotesThe Magna MeRIP M6A Kit provides sufficient reagents for 10 MeRIP reactions using 5 µg of mRNA or 5 MeRIP reactions when 300 µg of total RNA is used.

      Please read through the entire protocol and carefully plan your work before starting. The MeRIP method requires multiple steps and can be done over a two-day period or over multiple days. There are several stopping points to allow the method to be carried out over multiple days. The approximate time required for each step and potential stopping points are provided in the tables below.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels.
      Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size10 Assays
      Transport Information
      Supplemental Information