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48-618MAG MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay

96-well plate  96-well plate
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      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Akt (Total) Akt (Ser473) H, M, RMplexLuminex xMAP
      Catalogue Number48-618MAG
      Trade Name
      DescriptionMILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog # 48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
      Product Information
      Detection methodLuminex xMAP
      Panel TypeCell Signaling
      Quality LevelMQ200
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 2 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • Akt (Total)
      • Akt (Ser473)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information



      2-Plex Total/Phospho Akt Magnetic Bead Kit -96-well Plate Assay

      MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay SDS


      Safety Data Sheet (SDS) 


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      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Application Note: Bird¿s eye view of intracellular signal transduction pathways using customizable MILLIPLEX® MAP Cell Signaling Phospho/Total 2-Plex assays

      Data Sheet

      Full Service Custom-built Assays (MilliporeSigma)