Millipore Sigma Vibrant Logo

48-618MAG MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay

48-618MAG
96-well plate  96-well plate
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Akt (Total) Akt (Ser473) H, M, RMplexLuminex xMAP
      Description
      Catalogue Number48-618MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog # 48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 2 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • Akt (Total)
      • Akt (Ser473)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      2-Plex Total/Phospho Akt Magnetic Bead Kit -96-well Plate Assay

      MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Custom Immunoassay Menu of Services
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Application Note: Bird¿s eye view of intracellular signal transduction pathways using customizable MILLIPLEX® MAP Cell Signaling Phospho/Total 2-Plex assays

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)