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PRCYTA-40K MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A

PRCYTA-40K

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Description
Catalogue NumberPRCYTA-40K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition, each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background Information“Cytokine” is a general term used for a diverse group of small proteins, peptides or glycoproteins secreted by lymphocytes, monocytes, macrophages and other cells that regulate immune responses, haematopoiesis and lymphocyte development. Cytokines include interleukins, interferons, chemokines and other signaling molecules. Growth factors are extracellular polypeptides that have a positive effect on cell growth and proliferation. They can stimulate growth in a variety of different cell types. Growth factors and cytokines are similar in structure and the way of action. Individual cytokines or growth factors are produced by multiple cell types. This is different from hormones, which tend to be made by specialized glands. Each cytokine or growth factor acts through its own receptor on target cells to generate signaling pathways, and as a consequence to regulate biological processes. Some intracellular signaling components are shared between cytokines and growth factors. Expression of cytokines or growth factors and their receptors is highly regulated. De-regulation may contribute to many diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer. Cytokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and combating related diseases. As the most closely related species to humans, non-human primates are critical models for those studies.

The MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel is a 48-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples and tissue/cell lysate and culture supernatant samples: BCA-1, sCD137, sCD40L, Eotaxin, sFASL, FGF-2, Fractalkine, G-CSF, GM-CSF, Granzyme A, Granzyme B, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-15, IL-16, IL-17A, IL-17E, IL-18, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, MIP-3α, Perforin, RANTES, TGFα, TNFα, TNFβ, VEGF-A.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <20%
  • TNFα Inter-assay: <30%
Multiplex Ordering RequirementsNote: Sample type must be selected when configuring this kit. RANTES cannot be plexed with other analytes for serum/plasma and is not intended to be plexed into the premix kits when measuring serum/plasma samples. For questions, contact Technical Service.
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze up to 48 cytokine, chemokine, and growth factor biomarkers with a bead-based multiplex assay using Luminex® xMAP® technology, in non-human primate serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all of the Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
• RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples. For more information, please carefully review the sample preparation for serum and plasma samples and the preparation of antibody-immobilized beads sections below when assaying RANTES.
• The serum/plasma samples of some non-human primate species may require up to 1:100 dilution in Assay Buffer for IL-8.
Biological Information
Species Reactivity
  • Non-human Primate
Analytes Available
  • sCD40L
  • Eotaxin
  • FGF-2
  • Fractalkine
  • G-CSF
  • GM-CSF
  • IFNα2
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-15
  • IL-17A
  • IL-18
  • IL-22
  • IP-10
  • MCP-1
  • MIG
  • MIP-1β
  • RANTES
  • VEGF-A
  • BCA-1
  • sCD137
  • IL-16
  • Granzyme A
  • Granzyme B
  • IL-23
  • Perforin
  • sFasL
  • IL-28A
  • IL-31
  • IL-33
  • IL-17E/IL-25
  • IL-21
  • I-TAC
  • MIP-3α
  • TNFα
  • TGFα
  • MIP-1α
  • TNFβ
  • IL-1ra
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, except G-CSF had ~5% cross-reactivity with IL-1β.
Accuracy
  • 80 - 102%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • sCD40L: 48.83 - 200,000 pg/mL
  • BCA-1, Eotaxin, GM-CSF, IL-1α, IL-2, IL-17A, IL-6, IP-10, I-TAC, MIP-3α, TNFβ: 1.22 - 5,000 pg/mL
  • FGF-2: 61.04 - 250,000 pg/mL
  • sCD137, Granzyme B, IL-1β: 0.98 - 4,000 pg/mL
  • Fractalkine, IL-17E: 24.41 - 100,000 pg/mL
  • IFNγ, IFNα2, IL-10, IL-22, VEGF-A: 2.44 - 10,000 pg/mL
  • IL-16: 36.62 - 150,000 pg/mL
  • sFASL, G-CSF, IL-1RA, IL-21, IL-7, MIG, TNFα: 4.88 - 20,000 pg/mL
  • Granzyme A: 0.61 - 2,500 pg/mL
  • IL-15, MIP-1β: 6.10 - 25,000 pg/mL
  • IL-8: 0.05 - 200 pg/mL
  • IL-23, IL-18: 19.53 - 80,000 pg/mL
  • IL-12(p70), IL-28A, IL-31, IL-4: 12.21 - 50,000 pg/mL
  • IL-33: 14.65 - 60,000 pg/mL
  • IL-5: 2.93 - 12,000 pg/mL
  • MCP-1: 3.05 - 12,500 pg/mL
  • MIP-1α: 0.73 - 3,000
  • RANTES: 0.10 - 400
  • TGF-α: 0.49 - 2,000
  • Perforin: 97.66 - 400,000
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions• Recommended storage for kit components is 2 - 8°C.
• For long-term storage, freeze reconstituted standards and controls at -20°C. Avoid multiple freeze/thaw cycles.
• DO NOT FREEZE Antibody-Immobilized Beads, Detection Antibody, and Streptavidin-Phycoerythrin.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications

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