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RPTMAG-86K MILLIPLEX MAP Rat Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay

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RPTMAG-86K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ACTH BDNF FSH GH LH Prolactin TSH RMplexLuminex xMAP
Description
Catalogue NumberRPTMAG-86K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay
OverviewMILLIPLEX MAP Rat Pituitary Magnetic Bead Panel is used for the simultaneous quantification of the following 7 rat pituitary hormones: Growth Hormone (GH), Thyroid-Stimulating Hormone (TSH), Adrenocorticotropic Hormone (ACTH), Luteinizing Hormone (LH), Prolactin, Follicle-Stimulating Hormone (FSH), and Brain-Derived Neurotrophic Factor (BDNF).

This kit may be used for the analysis of all or any combination of the above pituitary hormones in rat serum or plasma samples, rat tissue extract, or cell/tissue culture supernatant samples.
Background InformationCalled the “master gland” because it controls many other endocrine glands, the pituitary gland secretes a number of key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Millipore’s Rat Pituitary Magnetic Bead Panel enables you to explore various aspects of reproduction, growth, metabolic homeostasis, and pituitary-related diseases such as acromegaly, growth hormone deficiency, diabetes insipidus and pituitary tumors.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay (%CV): <10
  • Inter-assay (%CV): <20
Panel TypeMAGNETIC Endocrine
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH.
Key Applications
  • Multiplexing
Biological Information
Species Reactivity
  • Rat
Analytes Available
  • ACTH
  • BDNF
  • FSH
  • GH
  • LH
  • Prolactin
  • TSH
Cross ReactivityAntibody pairs in the panel are specific and demonstrate no cross-reactivity (<0.1%) with other analytes in the panel except for LH which has ~5% cross-reactivity with antibody pair for TSH.
Accuracy
  • ACTH 88%
  • BDNF 102%
  • FSH 92%
  • GH 75%
  • LH 89%
  • Prolactin 85%
  • TSH 90%
Physicochemical Information
Sensitivity
  • ACTH 1.95 pg/mL
  • BDNF 0.38 pg/mL
  • FSH 7.62 pg/mL
  • GH 6.50 pg/mL
  • LH 3.28 pg/mL
  • Prolactin 4.64 pg/mL
  • TSH 0.87 pg/mL
Standard Curve Range
  • ACTH, BDNF, LH, TSH: 3.2–10,000 pg/mL
  • GH, Prolactin: 16–50,000 pg/mL
  • FSH: 32–100,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Rat Pituitary Magnetic Bead Panel

MILLIPLEX MAP Rat Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Effects of perinatal bisphenol A exposure during early development on radial arm maze behavior in adult male and female rats.
Sadowski, RN; Park, P; Neese, SL; Ferguson, DC; Schantz, SL; Juraska, JM
Neurotoxicology and teratology  42  17-24  2014

Show Abstract
24440629 24440629
Dynamic changes in fetal Leydig cell populations influence adult Leydig cell populations in mice.
Barsoum, IB; Kaur, J; Ge, RS; Cooke, PS; Yao, HH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology  27  2657-66  2013

Show Abstract
23568777 23568777
Global but not gonadotrope-specific disruption of Bmal1 abolishes the luteinizing hormone surge without affecting ovulation.
Chu, A; Zhu, L; Blum, ID; Mai, O; Leliavski, A; Fahrenkrug, J; Oster, H; Boehm, U; Storch, KF
Endocrinology  154  2924-35  2013

Show Abstract
23736292 23736292

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Analyte Quarterly Vol. 2 2020
Custom Immunoassay Menu of Services
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges: Milliplex Rat Pituitary and Milliplex Rat Pituitary Magnetic Kit

Data Sheet

Title
Full Service Custom-built Assays (MilliporeSigma)

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)