RECYTMAG-65K | MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

RECYTMAG-65K

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Overview

Replacement Information
Replacement InformationThis kit replaces RCYTOMAG-80K

Special Offers

This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
EGF, Eotaxin/CCL11, Fractalkine, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10, Leptin, LIX, MCP-1, MIP-1α, MIP-2, RANTES, TNF-α, VEGF R Mplex Luminex xMAP
Description
Catalogue NumberRECYTMAG-65K
ReplacesRCYTOMAG-80K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewThe MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel (RECYTMAG-65K) enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression.

References:
Neurosci Lett. 2015 Aug 18;602:120-5. doi: 10.1016/j.neulet.2015.06.040.
Neuroprotective effects of bisperoxovanadium on cerebral ischemia by inflammation inhibition.
Mao LL, Hao DL, Mao XW, Xu YF, Huang TT, Wu BN, Wang LH.

J Cereb Blood Flow Metab. 2016 Mar 16. pii: 0271678X16638669.
Delayed ALK5 inhibition improves functional recovery in neonatal brain injury.
Guardia Clausi M, Levison SW.
Background InformationThe immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-Assay: <25
  • Intra-Assay: <10
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in rat serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application NotesThis is a 2-hour room-temperature incubation assay requiring 25 μL 1:2 dilution rat serum or plasma samples.
Biological Information
Species Reactivity
  • Rat
Analytes Available
  • EGF
  • Eotaxin/CCL11
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GRO/KC
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-18
  • IP-10
  • Leptin
  • LIX
  • MCP-1
  • MIP-1α
  • MIP-2
  • RANTES
  • TNF-α
  • VEGF
Cross ReactivityThere is no or minimum cross-reactivity between the antibodies and any other analytes in the panel.
Accuracy
  • 80 - 131%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivites for individual cytokines/chemokines
Standard Curve Range
  • EGF: 0.2 – 1,000 pg/mL
  • Eotaxin, C-CSF, IL-4, IL-5, IL-13, RANTES, VEGF: 4.9 – 20,000 pg/mL
  • Fractalkine, IP-10, Leptin, TNFα: 2.4 – 10,000 pg/mL
  • GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18: 12.2 – 50,000 pg/mL
  • GRO/KC: 1.2 – 5,000 pg/mL
  • IFNγ, MIP-1α: 14.6 – 60,000 pg/mL
  • IL-6: 73.2 – 300,000 pg/mL
  • IL-10, IL-17A, MCP-1: 7.3 – 30,000 pg/mL
  • LIX, MIP-2: 24.4 – 100,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Rat Cytokine / Chemokine Magnetic Bead Panel

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
A guinea pig model of Zika virus infection.
Kumar M, Krause KK, Azouz F, Nakano E, Nerurkar VR.
Virol J.  2017

Show Abstract
28399888 28399888
α1-Antitrypsin inhibits ischemia reperfusion-induced lung injury by reducing inflammatory response and cell death.
Gao, W; Zhao, J; Kim, H; Xu, S; Chen, M; Bai, X; Toba, H; Cho, HR; Zhang, H; Keshavjeel, S; Liu, M
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation  33  309-15  2014

Show Abstract
24365768 24365768
The preclinical efficacy, selectivity and pharmacologic profile of MK-5932, an insulin-sparing selective glucocorticoid receptor modulator.
Brandish, PE; Anderson, K; Baltus, GA; Bai, C; Bungard, CJ; Bunting, P; Byford, A; Chiu, CS; Cicmil, M; Corcoran, H; Euler, D; Fisher, JE; Gambone, C; Hasbun-Manning, M; Kuklin, N; Landis, E; Lifsted, TQ; McElwee-Witmer, S; McIntosh, IS; Meissner, RS; Miao, J; Mitchell, HJ; Musselman, A; Schmidt, A; Shin, J; Szczerba, P; Thompson, CD; Tribouley, C; Vogel, RL; Warrier, S; Hershey, JC
European journal of pharmacology  724  102-11  2014

Show Abstract
24374007 24374007
Ginsenoside Rd maintains adult neural stem cell proliferation during lead-impaired neurogenesis.
Wang, B; Feng, G; Tang, C; Wang, L; Cheng, H; Zhang, Y; Ma, J; Shi, M; Zhao, G
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology  34  1181-8  2013

Show Abstract
23073826 23073826
Implications of time-series gene expression profiles of replicative senescence.
Kim, YM; Byun, HO; Jee, BA; Cho, H; Seo, YH; Kim, YS; Park, MH; Chung, HY; Woo, HG; Yoon, G
Aging cell  12  622-34  2013

Show Abstract
23590226 23590226

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Analyte Quarterly Vol 1 2018
Protein Detection Platform and Services Brochure

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Application Note: Drug-induced liver injury: Development of a MILLIPLEX® MAP multiplexed assay panel for emerging biomarkers (EMD)
Quality Control Ranges - Rat Cytokine/Chemokine Magnetic Panel
RECYTMAG-65K xPonent Protocol Template Version 3.1
RECYTMAG-65K xPonent Protocol Template Version Magpix
RECYTMAG-65K xPonent Standard Template Version 3.1
RECYTMAG-65K xPonent Standard Template Version Magpix

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)
MILLIPLEX® MAP Cell Signaling Wall Chart