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48-684MAG MILLIPLEX MAP Ras-Raf Oncoprotein Magnetic Bead Panel 6-Plex - Cell Signaling Multiplex Assay

48-684MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      BRAF (Ser446), CRAF (Ser338), MEK1 (Ser217/Ser221), Ras (total), RasG12D, RasG12V H Mplex Luminex xMAP
      Description
      Catalogue Number48-684MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Ras-Raf Oncoprotein Magnetic Bead Panel 6-Plex - Cell Signaling Multiplex Assay
      OverviewRas proteins are GTPases encoded by three genes: HRAS, KRAS, and NRAS. The protein products of these genes exhibit a high degree of sequence identity and are regulated by a conserved mechanism: exchange of GDP for GTP switches the Ras protein into an active conformation. In its GTP-bound state, Ras has high affinity for its downstream effectors, including the Raf family of protein kinases (ARAF, BRAF, and CRAF/RAF1). Raf proteins, in turn, signal through MEK to activate the MAPK pathway. In healthy cells, Ras activation is a transient response to extracellular growth stimuli and is restricted by the rapid conversion of Ras-GTP back to Ras-GDP. However, the Ras-Raf-MEK pathway is often aberrantly activated in cancer. Mutations in the RAS genes are amongst the most frequently encountered oncogenic events, occurring in ~25% of human cancers, with mutations altering the 12th amino acid (glycine) being particularly prevalent. These driver mutations maintain Ras in a constitutively active, GTP-bound state. Additionally, certain cancers feature KRAS gene amplification, although the effects of gene amplification on protein expression are not yet well-characterized.

      The MILLIPLEX® MAP 6-plex Ras-Raf Oncoprotein Magnetic Bead Kit (Cat. No. 48-684MAG) is a premixed kit to be used for the simultaneous quantification of the following 6 protein analytes:

      • pBRAF (Ser446)
      • pCRAF (Ser338)
      • pMEK1 (Ser217/Ser221)
      • Ras (total)
      • Mutant Ras protein, RasG12D*
      • Mutant Ras protein, RasG12V*
      *Detects H-Ras, N-Ras, and K-Ras protein
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay:< 15
      • Inter-assay: < 20
      Panel TypeCell Signaling
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This kit must be run using Assay Buffer 2 (provided).
      • This assay requires 25 µL diluted cell lysate per well.
      • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • BRAF (Ser446)
      • CRAF (Ser338)
      • MEK1 (Ser217/Ser221)
      • Ras (total)
      • RasG12D
      • RasG12V
      Physicochemical Information
      Standard Curve Range
      • 0.13–2,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications