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MSHMAG-21K MILLIPLEX MAP Multi-Species Hormone Magnetic Bead Panel

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Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Cortisol Estradiol Progesterone T3 T4 H, Ca, Multi-SpeciesMplexLuminex xMAP
Catalogue NumberMSHMAG-21K
Trade Name
DescriptionMILLIPLEX MAP Multi-Species Hormone Magnetic Bead Panel
OverviewThe MILLIPLEX® MAP Multi-Species Hormone Magnetic Bead Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Testosterone, Progesterone, T3 and T4.
Background InformationSteroid and thyroid hormones are small molecule endocrine hormones secreted from adrenal glands (cortisol), ovaries (estradiol and progesterone), and the thyroid (T3 and T4). These hormones play key roles in the regulation of energy balance, mental and physical development, sex organ development, reproduction and pregnancy. MilliporeSigma has developed a multiplex assay panel for simultaneous measurement of these hormones. This multiplex assay provides researchers with quality tools for the study of the biological functions of these hormones in the reproductive cycle and in the patho-physiology of many diseases such as thyroid-related diseases (Grave’s disease and hyper/hypothyroidism), steroid-related diseases (Cushing’s syndrome) and stress related diseases. This assay is a valuable tool for studying endocrine disrupting chemicals (EDC).
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Cortisol: Intra-assay <10% Inter-assay: < 10%
  • Estradiol: Intra-assay <10% Inter-assay: < 15%
  • Progesterone: Intra-assay <10% Inter-assay: < 15%
  • Testosterone: Intra-assay <10% Inter-assay: < 10%
  • T3: Intra-assay <10% Inter-assay: < 15%
  • T4: Intra-assay <10% Inter-assay: < 15%
Panel TypeMAGNETIC Endocrine
ApplicationThe Multi-Species Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 steroid/thyroid biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is a competitive immunoassay for multiplexing.
• This assay requires an extraction step for serum and plasma samples (instructions are provided in the kit protocol).
• 25 uL of extracted serum or plasma is used for each sample well.• Tissue/cell culture conditioned media/supernatants may be run neat or diluted in an appropriate control media.
• Each well requires 25 μL of sample, standard or control.
• For optimal sensitivity, use an overnight (16-18 hour) incubation.
Biological Information
SpecificityApproximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.

This multi-species kit may be used to detect these 5 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
Species Reactivity
  • Human
  • Canine
  • Multi-Species
Analytes Available
  • Cortisol
  • Estradiol
  • Progesterone
  • T3
  • T4
Assay PanelEverything you need in a single kit.
Cross ReactivityApproximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.
  • Human Samples:
  • Cortisol: 135%
  • Estradiol: 80%
  • Progesterone: 85%
  • T3: 107%
  • T4 97%
  • Canine Samples:
  • Cortisol: 117%
  • Estradiol: 80%
  • Progesterone: 67%
  • T3: 105%
  • T4: 87%
Physicochemical Information
  • MinDC+2SD
  • Cortisol: 0.33 ng/mL
  • Estradiol: 0.01 ng/mL
  • Progesterone: 0.25 ng/mL
  • T3: 0.12 ng/mL
  • T4: 0.51 ng/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Cortisol: 0.27 - 200 ng/mL
  • Estradiol: 0.014 - 10 ng/mL
  • Progesterone: 0.14 - 100 ng/mL
  • T3: 0.07 - 50 ng/mL
  • T4: 0.34 - 250 ng/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
MSHMAG-21K 04054839472879