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MT17MAG47K-PX25 MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology Multiplex Assay

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MT17MAG47K-PX25
96-well plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      CD40 Ligand GM-CSF IFN-γ IL-1β IL-2 IL-4 IL-5 IL-6 IL-10 IL-12 (p70) IL-13 IL-15 IL-17A IL-17E/IL-25 IL-17F IL-21 IL-22 IL-23 IL-27 IL-28B IL-31 IL-33 MIP-3α/CCL20 TNF-α TNFβ MMplexLuminex xMAP
      Description
      Catalogue NumberMT17MAG47K-PX25
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology Multiplex Assay
      OverviewThe MILLIPLEX MAP Mouse Th17 Magnetic Bead panel enables you to focus on the therapeutic potential of the modulation of CD4 T-helper cell cytokines. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
      Background InformationCD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFN, secrete IFN that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

      Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Inter-assay: < 15
      • Intra-assay:< 10
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      ApplicationSimultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is a two-hour or overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
      • This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Mouse
      Analytes Available
      • CD40 Ligand
      • GM-CSF
      • IFN-γ
      • IL-1β
      • IL-2
      • IL-4
      • IL-5
      • IL-6
      • IL-10
      • IL-12 (p70)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17E/IL-25
      • IL-17F
      • IL-21
      • IL-22
      • IL-23
      • IL-27
      • IL-28B
      • IL-31
      • IL-33
      • MIP-3α/CCL20
      • TNF-α
      • TNFβ
      Cross ReactivityThere was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol).
      Accuracy
      • 85 - 110%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities of individual biomarkers.
      Linearity of Dilution1:4 111.0% 1:8 113.1%
      Standard Curve Range
      • CD40L IL-31 & MIP-3α: 49 – 50,000 pg/mL
      • GM-CSF & IL-15: 34 – 35,000 pg/mL
      • IFNγ & IL-6: 7.8 – 8,000 pg/mL
      • IL-1β: 15 – 15,000 pg/mL
      • IL-2: 6.9 – 6,000 pg/mL
      • IL-4: 1.5 – 1,500 pg/mL
      • IL-5: 4.9 – 5,000 pg/mL
      • IL-10, IL-12(p70), & IL-21:20 – 20,000 pg/mL
      • IL-13 & IL-17A: 39 – 40,000 pg/mL
      • IL-17E/ IL-25: 586 – 600,000 pg/mL
      • IL-17F: 10 – 10,000 pg/mL
      • IL-22: 2.4 – 2,500 pg/mL
      • IL-23: 342 -350,000 pg/mL
      • IL-27: 879 – 900,000 pg/mL
      • IL-28B: 127 – 130,000 pg/mL
      • IL-33: 78 – 80,000 pg/mL
      • TNFβ:488 – 500,000 pg/mL
      • TNFα: 3.4 – 3,500 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Mouse Th17 Magnetic Bead Panel 96-Well

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Analyte Quarterly Vol. 2 2020
      Protein Detection Platform and Services Brochure
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Ranges - MILLIPLEX® MAP Mouse Th17 Magnetic Bead Panel

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)