MTH17MAG-47K MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay

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MTH17MAG-47K

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Overview

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Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-28B, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ M Mplex Luminex xMAP
Description
Catalogue NumberMTH17MAG-47K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay
OverviewThe MILLIPLEX MAP Mouse Th17 Magnetic Bead panel enables you to focus on the therapeutic potential of the modulation of CD4 T-helper cell cytokines. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background InformationCD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFN, secrete IFN that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay:< 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is a two-hour or overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • CD40 Ligand
  • GM-CSF
  • IFN-γ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17E/IL-25
  • IL-17F
  • IL-21
  • IL-22
  • IL-23
  • IL-27
  • IL-28B
  • IL-31
  • IL-33
  • MIP-3α/CCL20
  • TNF-α
  • TNFβ
Cross ReactivityThere was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol).
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Linearity of Dilution1:4 111.0% 1:8 113.1%
Standard Curve Range
  • CD40L IL-31 & MIP-3α: 49 – 50,000 pg/mL
  • GM-CSF & IL-15: 34 – 35,000 pg/mL
  • IFNγ & IL-6: 7.8 – 8,000 pg/mL
  • IL-1β: 15 – 15,000 pg/mL
  • IL-2: 6.9 – 6,000 pg/mL
  • IL-4: 1.5 – 1,500 pg/mL
  • IL-5: 4.9 – 5,000 pg/mL
  • IL-10, IL-12(p70), & IL-21:20 – 20,000 pg/mL
  • IL-13 & IL-17A: 39 – 40,000 pg/mL
  • IL-17E/ IL-25: 586 – 600,000 pg/mL
  • IL-17F: 10 – 10,000 pg/mL
  • IL-22: 2.4 – 2,500 pg/mL
  • IL-23: 342 -350,000 pg/mL
  • IL-27: 879 – 900,000 pg/mL
  • IL-28B: 127 – 130,000 pg/mL
  • IL-33: 78 – 80,000 pg/mL
  • TNFβ:488 – 500,000 pg/mL
  • TNFα: 3.4 – 3,500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Mouse Th17 Magnetic Bead Panel 96-Well

MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Analyte Quarterly Vol 1 2020
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
MTH17MAG-47K xPonent Protocol Template Version 3.1
MTH17MAG-47K xPonent Protocol Template Version Magpix
Quality Control Ranges - MILLIPLEX® MAP Mouse Th17 Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays (MilliporeSigma)

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)
MILLIPLEX® MAP Cell Signaling Wall Chart

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Categories

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits