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MECY2MAG-73KPX MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73KPX

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MECY2MAG-73KPX
96-well plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Erythropoietin (EPO) Exodus-2 Fractalkine/CX3CL1 IFNβ1 IFNγ IL-11 IL-16 IL-17A/F IL-20 MCP-5/CCL12 MDC (CCL22) MIP-3α/CCL20 MIP-3β/CCL19 TARC/CCL17 TIMP-1 MMplexLuminex xMAP
      Description
      Catalogue NumberMECY2MAG-73KPX
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73KPX
      OverviewThe MILLIPLEX® MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II Premixed 15 Plex, contains all the components necessary to measure the following 15 biomarkers using Luminex® xMAP® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.
      Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.
      References
      Product Information
      Components
      • Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized
      • Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized
      • Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized
      • Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
      • Assay Buffer, 1 bottle, 30 mL
      • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
      • Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL
      • Mouse Cytokine/Chemokine Panel II Premixed 15-Plex Beads, 3.5 mL
      • Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
      • Mixing Bottle, 1 bottle
      • Sample Plate, 96 well format, 1 plate
      • 2 Foil Plate Sealers
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Inter-assay: <20
      • Intra-assay: <10
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application NotesThis assay is an overnight assay (16 hour incubation) or same day assay (2 hour incubation). Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control. Tissue culture supernatants can also be used and if needed, make appropriate dilutions with control medium.
      Biological Information
      SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
      Species Reactivity
      • Mouse
      Analytes Available
      • Erythropoietin (EPO)
      • Exodus-2
      • Fractalkine/CX3CL1
      • IFNβ1
      • IFNγ
      • IL-11
      • IL-16
      • IL-17A/F
      • IL-20
      • MCP-5/CCL12
      • MDC (CCL22)
      • MIP-3α/CCL20
      • MIP-3β/CCL19
      • TARC/CCL17
      • TIMP-1
      Assay PanelEverything you need in a single kit.
      Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
      Accuracy
      • 77% – 105%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities of individual cytokines/chemokines.
      Linearity of DilutionContact Technical Support for this information.
      Standard Curve Range
      • Erythropoietin (EPO): 5 – 20,000 pg/mL
      • Exodus-2/CCL21/6Ckine: 12 – 50,000 pg/mL
      • Fractalkine/CX3CL1: 24 – 100,000 pg/mL
      • IFNβ1: 12 – 50,000 pg/mL
      • IFNγ: 1 – 5,000 pg/mL
      • IL-11: 12 – 50,000 pg/mL
      • IL-16: 2 – 10,000 pg/mL
      • IL-17A/F: 24 – 100,000 pg/mL
      • IL-20: 12 – 50,000 pg/mL
      • MCP-5/CCL12: 2 – 10,000 pg/mL
      • MDC/CCL22: 2 – 10,000 pg/mL
      • MIP-3α/CCL20: 2 – 10,000 pg/mL
      • MIP-3β/CCL19: 12 – 50,000 pg/mL
      • TARC/CCL17: 2 – 10,000 pg/mL
      • TIMP-1: 2 – 10,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications