HNS2MAG-95K MILLIPLEX MAP Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay

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HNS2MAG-95K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP, TREM2 H Mplex Luminex xMAP
Description
Catalogue NumberHNS2MAG-95K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay
OverviewEMD Millipore’s MILLIPLEX® MAP Human Neuroscience Magnetic Bead Panel 2 is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF), serum/plasma and cell/tissue culture supernatants for homogenates: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP, TREM2
Background InformationMonitoring protein biomarkers in cerebrospinal fluid (CSF) of patients with neurological disorders has been highly beneficial to understanding disease progression. While several CSF biomarkers can reproducibly distinguish normal and diseased samples, CSF is a difficult biological fluid to obtain in research studies. The need for blood-based biomarkers of AD has driven a continuous search for novel candidates. This configurable multiplex immunoassay quantitatively measures seven proteins present in both human CSF and blood plasma/serum that are involved in neurological disease. Cell/tissue culture samples may also be used in the panel.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Angiogenin: Intra-assay:< 5% CV, Inter-assay: < 10% CV
  • ApoE4: Intra-assay: < 5% CV, Inter-assay: <15% CV
  • FABP3: Intra-assay: < 5% CV, Inter-assay: < 10% CV
  • Ferritin: Intra-assay: < 10% CV, Inter-assay: < 15% CV
  • Neurogranin: Intra-assay: < 10% CV, Inter-assay: < 15% CV
  • PRNP: Intra-assay: < 5% CV, Inter-assay: < 10% CV
  • TREM2: Intra-assay: <5% CV, Inter-assay: < 10% CV
Panel TypeMAGNETIC Neuroscience
Applications
ApplicationThe analytes available for this multiplex kit are: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP and TREM2.
Key Applications
  • Multiplexing
Application Notes• For Plasma/Serum a 1:10 dilution is recommended for the optimal detection of the analytes FABP3, Ferritin, Neurogranin, PRNP and TREM2.
• For Plasma/Serum a 1:200 dilution for ApoE4 and Angiogenin is recommended.
• Because the sample dilutions differ for Plasma/Serum samples ApoE4 and Angiogenin cannot be plexed in the same assay as FABP3, Ferritin, Neurogranin, PRNP and TREM2.
• CSF samples require a 1:10 dilution and so may all be plexed together
• Cell/Tissue culture supernatants or homogenates may be used neat or diluted in appropriate control medium.
• Bead diluent must be present in order to detect Angiogenin. (Biotinylated-Angiogenin detection antibody is included in the bead diluent.)
• This is an overnight (2-8C) assay requiring 25 ul of appropriately diluted sample per well. Alternative, a 2 hour room temperature (20-25C) incubation may be used, thought assay sensitivity may be lower.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Angiogenin
  • ApoE4
  • FABP3
  • Ferritin
  • Neurogranin
  • PRNP
  • TREM2
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. There is <5% cross-reactivity from ApoE2 and ApoE3 recombinant proteins on the ApoE4 beads.
Accuracy
  • Angiogenin 97%
  • ApoE4 85%
  • FABP3 84%
  • Ferritin 98%
  • Neurogranin 124%
  • PRNP 96%
  • TREM2 94%
Physicochemical Information
Sensitivity
  • MinDC + 2SD (pg/mL)
  • Angiogenin: 5.8
  • ApoE4: 162.8
  • FABP3: 14.3
  • Ferritin: 6.1
  • Neurogranin: 19.9
  • PRNP: 177.0
  • TREM2: 11.0
Standard Curve Range
  • Angiogenin: 2 - 10,000 pg/mL
  • ApoE4: 244 - 1,000,000 pg/mL
  • FABP3: 24 - 100,000 pg/mL
  • Ferritin: 6 - 25,000 pg/mL
  • Neurogranin: 5 - 20,000 pg/mL
  • PRNP: 122 - 500,000 pg/mL
  • TREM2: 15 - 50,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications