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HGAMMAG-303E MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay

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HGAMMAG-303E
96-well plate  96 Well Plate
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      IgE HMplexLuminex xMAP
      Description
      Catalogue NumberHGAMMAG-303E
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
      Background InformationFound only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found
      primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.

      Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
      References
      Product Information
      Components
      • MILLIPLEX® MAP Anti-Human k and λ Light Chain, PE
      • MILLIPLEX® MAP Human IgE Standard
      • MILLIPLEX® MAP Human Serum Positive Control
      • MILLIPLEX® MAP Assay Buffer
      • MILLIPLEX® MAP Washer Buffer, 10X
      • Set of one 96-Well Filter Plate with 4 Sealers
      Detection methodLuminex xMAP
      ConfigurationSingle Plex
      Precision, %
      • Intra-Assay < 10
      • Inter-Assay < 13
      Panel TypeMAGNETIC Immune Response
      Quality LevelMQ200
      Applications
      ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This kit may be used for the analysis of IgE in human serum, plasma or tissue/cell lysate and culture supernatant samples.
      • There are 2 one-hour room-temperature incubations.
      • This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well.
      Biological Information
      SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • IgE
      Accuracy
      • IgE 102%
      Physicochemical Information
      Sensitivity
      • 0.4 ng/mL
      Standard Curve Range
      • IgE 4.1 – 1,000 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 Well Plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      MILLIPLEX MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit

      MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Custom Immunoassay Menu of Services
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      HGAMMAG-303E xPonent Protocol Template Version Magpix
      HGAMMAG-303E xPonent Standard Template Version Magpix
      Quality Control Ranges: Milliplex MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)